BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K531010
1
BBa_K531010
Constitutive promoter and RBS BBa_K081005 + <i>Caulobacter</i> optimized <i>dspB</i> + <i>rsaA</i> C
2011-09-22T11:00:00Z
2015-05-08T01:12:36Z
BBa_K081005 was designed by Lorenzo Pasotti and Paolo Magni from Team UNIPV-Pavia 2008. IDT synthesized <i>dspB</i> so as to codon optimize it for expression in <i>Caulobacter crescentus</I>. The C-terminal secretion tag from <i>rsaA</i> is from the genome of the <i>Caulobacter</i> lab strain CB15N.
Constitutive promoter and RBS from the Registry BBa_K081005, suspected N-acetylglucosaminidase <i>dspB</i> codon optimized for <I>Caulobacter</i>, and the C-terminal secretion tag from <I>Caulobacter's</i> S-layer associated protein, RsaA.
false
false
_697_
0
8840
9
Not in stock
false
Stop codon at the end of <i>dspB</i> was removed to ensure expression of the <i>rsaA</i> C-terminal secretion tag.
false
Alexander Aaring
component2156669
1
BBa_K531001
component2156667
1
BBa_K081005
component2156668
1
BBa_K531004
annotation2156667
1
BBa_K081005
range2156667
1
1
58
annotation2156668
1
BBa_K531004
range2156668
1
67
1151
annotation2156669
1
BBa_K531001
range2156669
1
1160
1519
BBa_K531004
1
BBa_K531004
dspB from Actinobacillus actinomycetemcomitansis optimized for Caulobacter crescentus
2011-07-10T11:00:00Z
2015-05-08T01:12:36Z
Kaplan, J., Ragunath, C., Ramasubbu, N., and Fine, D. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology. 2003 August; 185(16): 4693-4698.
DspB comes from the bacteria Actinobacillus actinomycetemcomitansis. It is most likely an N-acetylglucosaminidase that causes the detachment of cells from biofilm colonies through the 1→ 4 glycosidic bond of β-substituted N-acetylglucosamine. DspB is homologous to family 20 glycosyl hydrolases which cleave terminal monosaccharide residues.
false
false
_697_
0
9994
9
Not in stock
false
The WT gene has a low GC content ~40%. Caulobacter crescentus is GC rich and prefers a high GC content. Therefore, we optimized the GC content by comparing the codons in the wild type dspB gene to their corresponding amino acid codons that are most expressed in Caulobacter.
We also eliminated the stop codon at the end of dspB because we wanted to add another gene to it that also needed to be transcribed.
false
Nora Kostow
BBa_K531001
1
BBa_K531001
<i>C. crescentus</i> S-layer RsaA C-term
2011-07-03T11:00:00Z
2015-05-08T01:12:36Z
This comes from genomic ''Caulobacter'' CB15N.
This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag.
false
false
_697_
0
8840
9
In stock
true
Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence.
false
Alexander Aaring
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K081005
1
BBa_K081005
constitutive promoter family member and RBS
2008-10-17T11:00:00Z
2015-05-08T01:08:34Z
Promoter: John Anderson.
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Released HQ 2013
Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
false
true
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component1981865
1
BBa_B0030
component1981863
1
BBa_J23100
annotation1981863
1
BBa_J23100
range1981863
1
1
35
annotation1981865
1
BBa_B0030
range1981865
1
44
58
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K081005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K531010_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagagtgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K531001_sequence
1
gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K531004_sequence
1
tgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z