LOCUS       BBa_K537010              960 bp    DNA     linear       13-Jun-2024
DEFINITION  Promoter-Theophylline riboswitch 2-Venus-Double terminator
ACCESSION   BBa_K537010
VERSION     BBa_K537010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             86..90
                     /label=rbs
     misc_feature    101..103
                     /label=start
     misc_feature    44..103
                     /label=Theophylline riboswitch (type 2)
     misc_feature    44..69
                     /label=Aptamer
     CDS             101..823
                     /label=Venus
     misc_feature    44..823
                     /label=BBa_K537004
     stem_loop       843..886
                     /label=stem_loop
     misc_feature    832..911
                     /label=BBa_B0010
     terminator      832..911
                     /label=BBa_B0010
     stem_loop       927..946
                     /label=T7 TE
     misc_feature    953..953
                     /label=stop
     polyA_site      947..960
                     /label=polya
     misc_feature    920..960
                     /label=BBa_B0012
     terminator      920..960
                     /label=BBa_B0012
     terminator      832..960
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagggtgata ccagcatcgt
       61 cttgatgccc ttggcagcac cctgctaagg taacaacaag atggtttcaa agggtgaaga
      121 attatttact ggtgttgttc caattttagt tgaattagat ggtgatgtta acggtcacaa
      181 gttttcagtt tcaggtgaag gtgaaggtga tgctacttac ggtaagttaa ctttaaagtt
      241 aatttgtact actggtaagt taccagttcc atggccaact ttagttacta ctttaggtta
      301 cggtttacaa tgttttgctc gttacccaga tcacatgaag caacacgatt tttttaagtc
      361 agctatgcca gaaggttacg ttcaagaacg tactattttt tttaaggatg atggtaacta
      421 caagactcgt gctgaagtta agtttgaagg tgatacttta gttaaccgta ttgaattaaa
      481 gggtattgat tttaaggaag atggtaacat tttaggtcac aagttagaat acaactacaa
      541 ctcacacaac gtttacatta ctgctgataa gcaaaagaac ggtattaagg ctaactttaa
      601 gattcgtcac aacattgaag atggtggtgt tcaattagct gatcactacc aacaaaacac
      661 tccaattggt gatggtccag ttttattacc agataaccac tacttatcat accaatcaaa
      721 gttatcaaag gatccaaacg aaaagcgtga tcacatggtt ttattagaat ttgttactgc
      781 tgctggtatt actttaggta tggatgaatt atacaagtaa taatactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//