LOCUS       BBa_K538200              523 bp    DNA     linear       14-May-2024
DEFINITION  pLacI + RBS + Cpn10
ACCESSION   BBa_K538200
VERSION     BBa_K538200.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    518..523
                     /label=Double Stop Codon
     CDS             227..523
                     /label=BBa_K538000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaatccgtcc
      241 attacatgat cgtattgttg ttcgccgtaa agaagaagag accgcaactg cgggtggtat
      301 tattttaccg ggcgctgcgg cagaaaaacc aaatcaaggt gttgttatct ctgtgggtac
      361 tggccgtatt cttgataatg gttcagtgca agcgctggcg gttaacgaag gcgatgttgt
      421 cgtttttggt aaatactcag gtcaaaatac tatcgatatc gatggtgaag aattattgat
      481 tttgaatgaa agtgatatct acggcgtttt agaagcttaa taa
//