LOCUS       BBa_K538201             1876 bp    DNA     linear       15-May-2024
DEFINITION  pLacI + RBS + Cpn60
ACCESSION   BBa_K538201
VERSION     BBa_K538201.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    1871..1876
                     /label=Double Stop Codon
     misc_feature    1134..1134
                     /label=A > G (Recode for SapI site)
     CDS             227..1876
                     /label=BBa_K538001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgctaaaga
      241 cgtattattt ggtgatagcg cacgcgcaaa aatgttggta ggtgtaaaca ttttagccga
      301 cgcagtaaga gttaccttag gacctaaagg tcgtaacgtt gttatagaaa aatcatttgg
      361 tgcaccgatc atcaccaaag atggtgtttc tgttgcgcgt gaaatcgaat tgaaagacaa
      421 attcgaaaac atgggcgcac agatggttaa ggaagttgct tctcaagcca acgaccaagc
      481 cggtgacggc acaacgacag cgactgtact agcacaggcg attatcagcg aaggcttgaa
      541 atctgttgcg gctggcatga atccaatgga tcttaaacgt ggtattgata aagctacggc
      601 tgctgttgtt gccgccatta aagaacaagc tcagccttgc ttggatacaa aagcaatcgc
      661 tcaggtaggg acaatctctg ccaatgccga tgaaacggtt ggtcgtttaa ttgctgaagc
      721 gatggaaaaa gtcggtaaag aaggtgtgat taccgttgaa gaaggcaaag gccttgaaga
      781 cgagcttgat gttgtagaag gcatgcagtt cgatcgcggt tacttgtctc cgtacttcat
      841 caacaaccaa gaaaaaatga ccgtagaaat ggaaaatcca ttaattctat tggttgataa
      901 gaaaattgat aaccttcaag agctgttgcc aattcttgaa aacgtcgcta aatcaggtcg
      961 tccattattg atcgttgctg aagatgttga aggccaagca ctagcaacat tggtagtaaa
     1021 caacttgcgc ggcacattca aggttgcagc ggttaaagcc cctggttttg gcgatcgtcg
     1081 taaagcgatg ttgcaagatc ttgccatctt gacgggtggt caggttattt ctgaggagct
     1141 agggatgtct ttagaaactg cggatccttc ttctttgggt acggcaagca aggttgttat
     1201 cgataaagaa aacaccgtga ttgttgatgg cgcaggtact gaagcaagcg ttaatactcg
     1261 tgttgaccag atccgtgctg aaatcgaaag ctcgacttct gattacgaca tcgaaaagtt
     1321 acaagaacgc gttgctaagc ttgcgggcgg cgttgccgtg attaaggttg gtgcgggttc
     1381 tgaaatggaa atgaaagaga agaaagaccg tgttgacgat gcacttcatg caactcgcgc
     1441 agcggttgaa gaaggtgttg ttgcgggtgg tggtgttgct ttgattcgcg cactctcttc
     1501 agtaaccgtt gttggtgata acgaagatca aaacgtcggt attgcattgg cacttcgtgc
     1561 gatggaagct cctatccgtc aaatcgcggg taacgcaggt gctgaagggt cagtggttgt
     1621 tgataaagtg aaatctggca caggtagctt tggttttaac gccagcacag gtgagtatgg
     1681 cgatatgatt gcgatgggta ttttagaccc tgcaaaagtc acgcgttcat ctctacaagc
     1741 cgcggcgtct atcgcaggtt tgatgatcac aaccgaagcc atggttgcgg atgcgcctgt
     1801 tgaagaaggc gctggtggta tgcctgatat gggcggcatg ggtggaatgg gcggtatgcc
     1861 tggcatgatg taataa
//