LOCUS       BBa_K538202             2146 bp    DNA     linear       15-May-2024
DEFINITION  pLacI + RBS + SheDnaK
ACCESSION   BBa_K538202
VERSION     BBa_K538202.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    2141..2146
                     /label=Double Stop Codon
     misc_feature    571..571
                     /label=A > G (Recode for PvuII site)
     misc_feature    952..952
                     /label=A > G (Recode for EcoRI site)
     misc_feature    1171..1171
                     /label=T > G (Recode for XbaI site)
     misc_feature    1507..1507
                     /label=A > G (Recode for PvuII site)
     misc_feature    1897..1897
                     /label=T > G (Recode for NheI site)
     CDS             227..2146
                     /label=BBa_K538002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gcaaaattat
      241 tggtatcgat ttaggcacaa caaactcgtg tgtagcagtc cttgatggcg gcaaagcacg
      301 cgtaattgaa aacgcagagg gtgatcgtac aaccccatca attatcgctt ataccgatga
      361 tgaaattatt gtaggccaac cagcaaagcg tcaagctgta actaacccaa caaacacatt
      421 ctttgccatt aagcgtttaa tcggccgtcg ttttaaagat gacgaagtac aacgtgacgt
      481 gaacatcatg ccattcaaaa ttatcggagc tgataatggt gatgcatggg ttgaatcacg
      541 tggtaataaa atggcaccac cacaagtttc ggctgaaatc ttgaaaaaga tgaaaaagac
      601 tgctgaagac tttttaggtg aagaagtgac tgaagcggtt attaccgttc ctgcttactt
      661 taacgattca caacgtcaag caacgaaaga tgctggtcgt atcgcaggtc ttgatgttaa
      721 gcgtattatc aacgaaccta ctgctgctgc acttgcatac ggtatcgata agaagcaagg
      781 cgacaacatt gttgctgtat acgatttagg tggtggtaca ttcgatattt ctatcatcga
      841 aatcgacagt aacgatggcg accaaacatt tgaagtacta gcaaccaatg gtgatactca
      901 cttaggtggt gaagactttg ataaccgtat gattaactat ttagctgatg agttcaaaaa
      961 agaccagggc ttagatcttc gtagagatcc tttagcaatg caacgtttga aagaagcggc
     1021 tgaaaaagca aaaatcgagc tttcaagcac taaccacact gaagttaact tgccttacat
     1081 cactgctgat gcatcaggtc ctaagcattt agtggttaaa attactcgtg ctaagttaga
     1141 gtcattagtt gaagatttaa ttcaacgtac gctagagccg cttaaagttg cactagctga
     1201 tgctgattta tcaatatctg atatcaatga agtgattctt gttggtggcc agactcgtat
     1261 gcctaaagta caagaagcag taactaactt cttcggcaaa gagcctcgta aagatgttaa
     1321 ccctgatgaa gcggttgcag ttggcgcggc gattcaggct ggcgtacttt ctggtgaagt
     1381 gaaagacgta cttctacttg acgttacacc actatctctt ggtattgaaa ccatgggcag
     1441 tgtgatgacc aagcttatcg agaagaacac cactatcccg actaaagctc agcaagtatt
     1501 ctcaacggct gacgacaacc aaagtgcagt gacaattcac gtacttcaag gtgagcgtaa
     1561 gcaagctagt gccaacaagt cattaggtca atttaaccta gaaggtattg agccagcacc
     1621 acgtggccaa ccacaggttg aagtgatgtt cgacattgat gctgatggta tcttacatgt
     1681 gtctgcaaca gacaagaaaa caggtaagaa acaaaatatt actatcaaag cttcttcagg
     1741 tttgtctgat gaagaagttg aacaaatggt acgtgatgca gaagctcatg ctgatgaaga
     1801 tgcgaaattt gaagagttag ttcaagcgcg taatcaagca gatggtttag ctcattcaac
     1861 taaaaaacaa gttgaagaag ctggcgatgc actagcgagc gacgaaaaag aaaagattga
     1921 agcggcaatt gcaactttag aaactgccat aaaaggcaaa gataaagaag ctattgatac
     1981 agctactcaa gcgttaatcg aagcgtctgc taagttaatg gaaattgctc aagctaaagc
     2041 tcaaggtgaa gcagaaggtc aagcacacga tgctggccaa gaaaagcctg ctgatgatgt
     2101 tgttgatgct gagttcgaag aagttaaaga cgacaaaaag taataa
//