BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_K538002
1
SheDnaK
SheDnaK (Shewanella sp. Ac10)
2011-07-28T11:00:00Z
2015-05-08T01:12:36Z
-
-
false
false
_704_
0
9985
9
Not in stock
false
-
false
Bas Stringer (Sequence by Paul van Dieken)
annotation2124233
1
Start Codon
range2124233
1
1
3
annotation2127787
1
A > G (Recode for PvuII site)
range2127787
1
345
345
annotation2127790
1
T > G (Recode for XbaI site)
range2127790
1
945
945
annotation2127789
1
A > G (Recode for EcoRI site)
range2127789
1
726
726
annotation2124234
1
Double Stop Codon
range2124234
1
1915
1920
annotation2127792
1
T > G (Recode for NheI site)
range2127792
1
1671
1671
annotation2127791
1
A > G (Recode for PvuII site)
range2127791
1
1281
1281
BBa_K538202
1
LacI:SDnaK
pLacI + RBS + SheDnaK
2011-09-07T11:00:00Z
2015-05-08T01:12:37Z
-
-
false
false
_704_
0
9985
9
Not in stock
false
-
false
Bas Stringer
component2126753
1
BBa_K538002
component2126742
1
BBa_R0010
component2126750
1
BBa_B0034
annotation2126750
1
BBa_B0034
range2126750
1
209
220
annotation2126742
1
BBa_R0010
range2126742
1
1
200
annotation2126753
1
BBa_K538002
range2126753
1
227
2146
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K538002_sequence
1
atgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaattgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagcaaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgacgaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtggtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtgaagaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgtatcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaaggcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatggcgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaactatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaagcggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatcaggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgctagagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccagactcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagcggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacaccactatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctcagcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctagtgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatgttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatcaaagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaaatttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggcgatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagataaagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctcaaggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaagttaaagacgacaaaaagtaataa
BBa_K538202_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaattgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagcaaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgacgaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtggtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtgaagaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgtatcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaaggcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatggcgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaactatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaagcggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatcaggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgctagagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccagactcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagcggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacaccactatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctcagcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctagtgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatgttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatcaaagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaaatttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggcgatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagataaagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctcaaggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaagttaaagacgacaaaaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z