LOCUS       BBa_K538204              421 bp    DNA     linear       15-May-2024
DEFINITION  pLacI + RBS + CspC
ACCESSION   BBa_K538204
VERSION     BBa_K538204.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    416..421
                     /label=Double Stop Codon
     misc_feature    301..301
                     /label=A > G (Recode for SapI site)
     misc_feature    263..280
                     /label=RNA binding motif (RPN1)
     misc_feature    305..319
                     /label=RNA binding motif (RPN2)
     CDS             227..421
                     /label=BBa_K538004
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ataaaggtac
      241 cgtaaaattt ttcaatgaat ctaaaggatt cggatttatc actgaagaag gaaacaatga
      301 ggagcatttt gtgcacgtgt caggattagt agacgaaatt cgtgaaaacg atgaggttga
      361 atttgactta caagatggaa gaaaaggatt aaacgcagta aacgtaagag ttatataata
      421 a
//