LOCUS       BBa_K538301             1806 bp    DNA     linear       16-May-2024
DEFINITION  pBAD + RBS + Cpn60
ACCESSION   BBa_K538301
VERSION     BBa_K538301.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start Codon
     misc_feature    1801..1806
                     /label=Double Stop Codon
     misc_feature    1064..1064
                     /label=A > G (Recode for SapI site)
     CDS             157..1806
                     /label=BBa_K538001
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg ctgctaaaga cgtattattt
      181 ggtgatagcg cacgcgcaaa aatgttggta ggtgtaaaca ttttagccga cgcagtaaga
      241 gttaccttag gacctaaagg tcgtaacgtt gttatagaaa aatcatttgg tgcaccgatc
      301 atcaccaaag atggtgtttc tgttgcgcgt gaaatcgaat tgaaagacaa attcgaaaac
      361 atgggcgcac agatggttaa ggaagttgct tctcaagcca acgaccaagc cggtgacggc
      421 acaacgacag cgactgtact agcacaggcg attatcagcg aaggcttgaa atctgttgcg
      481 gctggcatga atccaatgga tcttaaacgt ggtattgata aagctacggc tgctgttgtt
      541 gccgccatta aagaacaagc tcagccttgc ttggatacaa aagcaatcgc tcaggtaggg
      601 acaatctctg ccaatgccga tgaaacggtt ggtcgtttaa ttgctgaagc gatggaaaaa
      661 gtcggtaaag aaggtgtgat taccgttgaa gaaggcaaag gccttgaaga cgagcttgat
      721 gttgtagaag gcatgcagtt cgatcgcggt tacttgtctc cgtacttcat caacaaccaa
      781 gaaaaaatga ccgtagaaat ggaaaatcca ttaattctat tggttgataa gaaaattgat
      841 aaccttcaag agctgttgcc aattcttgaa aacgtcgcta aatcaggtcg tccattattg
      901 atcgttgctg aagatgttga aggccaagca ctagcaacat tggtagtaaa caacttgcgc
      961 ggcacattca aggttgcagc ggttaaagcc cctggttttg gcgatcgtcg taaagcgatg
     1021 ttgcaagatc ttgccatctt gacgggtggt caggttattt ctgaggagct agggatgtct
     1081 ttagaaactg cggatccttc ttctttgggt acggcaagca aggttgttat cgataaagaa
     1141 aacaccgtga ttgttgatgg cgcaggtact gaagcaagcg ttaatactcg tgttgaccag
     1201 atccgtgctg aaatcgaaag ctcgacttct gattacgaca tcgaaaagtt acaagaacgc
     1261 gttgctaagc ttgcgggcgg cgttgccgtg attaaggttg gtgcgggttc tgaaatggaa
     1321 atgaaagaga agaaagaccg tgttgacgat gcacttcatg caactcgcgc agcggttgaa
     1381 gaaggtgttg ttgcgggtgg tggtgttgct ttgattcgcg cactctcttc agtaaccgtt
     1441 gttggtgata acgaagatca aaacgtcggt attgcattgg cacttcgtgc gatggaagct
     1501 cctatccgtc aaatcgcggg taacgcaggt gctgaagggt cagtggttgt tgataaagtg
     1561 aaatctggca caggtagctt tggttttaac gccagcacag gtgagtatgg cgatatgatt
     1621 gcgatgggta ttttagaccc tgcaaaagtc acgcgttcat ctctacaagc cgcggcgtct
     1681 atcgcaggtt tgatgatcac aaccgaagcc atggttgcgg atgcgcctgt tgaagaaggc
     1741 gctggtggta tgcctgatat gggcggcatg ggtggaatgg gcggtatgcc tggcatgatg
     1801 taataa
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