LOCUS       BBa_K538302             2076 bp    DNA     linear       14-May-2024
DEFINITION  pBAD + RBS + SheDnaK
ACCESSION   BBa_K538302
VERSION     BBa_K538302.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start Codon
     misc_feature    2071..2076
                     /label=Double Stop Codon
     misc_feature    501..501
                     /label=A > G (Recode for PvuII site)
     misc_feature    882..882
                     /label=A > G (Recode for EcoRI site)
     misc_feature    1101..1101
                     /label=T > G (Recode for XbaI site)
     misc_feature    1437..1437
                     /label=A > G (Recode for PvuII site)
     misc_feature    1827..1827
                     /label=T > G (Recode for NheI site)
     CDS             157..2076
                     /label=BBa_K538002
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg gcaaaattat tggtatcgat
      181 ttaggcacaa caaactcgtg tgtagcagtc cttgatggcg gcaaagcacg cgtaattgaa
      241 aacgcagagg gtgatcgtac aaccccatca attatcgctt ataccgatga tgaaattatt
      301 gtaggccaac cagcaaagcg tcaagctgta actaacccaa caaacacatt ctttgccatt
      361 aagcgtttaa tcggccgtcg ttttaaagat gacgaagtac aacgtgacgt gaacatcatg
      421 ccattcaaaa ttatcggagc tgataatggt gatgcatggg ttgaatcacg tggtaataaa
      481 atggcaccac cacaagtttc ggctgaaatc ttgaaaaaga tgaaaaagac tgctgaagac
      541 tttttaggtg aagaagtgac tgaagcggtt attaccgttc ctgcttactt taacgattca
      601 caacgtcaag caacgaaaga tgctggtcgt atcgcaggtc ttgatgttaa gcgtattatc
      661 aacgaaccta ctgctgctgc acttgcatac ggtatcgata agaagcaagg cgacaacatt
      721 gttgctgtat acgatttagg tggtggtaca ttcgatattt ctatcatcga aatcgacagt
      781 aacgatggcg accaaacatt tgaagtacta gcaaccaatg gtgatactca cttaggtggt
      841 gaagactttg ataaccgtat gattaactat ttagctgatg agttcaaaaa agaccagggc
      901 ttagatcttc gtagagatcc tttagcaatg caacgtttga aagaagcggc tgaaaaagca
      961 aaaatcgagc tttcaagcac taaccacact gaagttaact tgccttacat cactgctgat
     1021 gcatcaggtc ctaagcattt agtggttaaa attactcgtg ctaagttaga gtcattagtt
     1081 gaagatttaa ttcaacgtac gctagagccg cttaaagttg cactagctga tgctgattta
     1141 tcaatatctg atatcaatga agtgattctt gttggtggcc agactcgtat gcctaaagta
     1201 caagaagcag taactaactt cttcggcaaa gagcctcgta aagatgttaa ccctgatgaa
     1261 gcggttgcag ttggcgcggc gattcaggct ggcgtacttt ctggtgaagt gaaagacgta
     1321 cttctacttg acgttacacc actatctctt ggtattgaaa ccatgggcag tgtgatgacc
     1381 aagcttatcg agaagaacac cactatcccg actaaagctc agcaagtatt ctcaacggct
     1441 gacgacaacc aaagtgcagt gacaattcac gtacttcaag gtgagcgtaa gcaagctagt
     1501 gccaacaagt cattaggtca atttaaccta gaaggtattg agccagcacc acgtggccaa
     1561 ccacaggttg aagtgatgtt cgacattgat gctgatggta tcttacatgt gtctgcaaca
     1621 gacaagaaaa caggtaagaa acaaaatatt actatcaaag cttcttcagg tttgtctgat
     1681 gaagaagttg aacaaatggt acgtgatgca gaagctcatg ctgatgaaga tgcgaaattt
     1741 gaagagttag ttcaagcgcg taatcaagca gatggtttag ctcattcaac taaaaaacaa
     1801 gttgaagaag ctggcgatgc actagcgagc gacgaaaaag aaaagattga agcggcaatt
     1861 gcaactttag aaactgccat aaaaggcaaa gataaagaag ctattgatac agctactcaa
     1921 gcgttaatcg aagcgtctgc taagttaatg gaaattgctc aagctaaagc tcaaggtgaa
     1981 gcagaaggtc aagcacacga tgctggccaa gaaaagcctg ctgatgatgt tgttgatgct
     2041 gagttcgaag aagttaaaga cgacaaaaag taataa
//