LOCUS       BBa_K539281             1605 bp    DNA     linear       28-May-2024
DEFINITION  rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI 
            regulater
ACCESSION   BBa_K539281
VERSION     BBa_K539281.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=cIts
     CDS             56..769
                     /label=BBa_K098997
     misc_feature    44..769
                     /label=BBa_K098998
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    778..929
                     /label=BBa_S04136
     misc_feature    44..929
                     /label=BBa_S04137
     misc_feature    1..929
                     /label=BBa_K098995
     RBS             938..953
                     /label=BBa_I742156
     CDS             954..1481
                     /label=crtZ
     CDS             954..1484
                     /label=BBa_I742157
     misc_feature    938..1484
                     /label=BBa_I742158
     terminator      1493..1605
                     /label=BBa_J61048
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagctc taccggagaa attatgttgt
      961 ggatttggaa tgccctgatc gttttcgtta ccgtgattgg catggaagtg attgctgcac
     1021 tggcacacaa atacatcatg cacggctggg gttggggatg gcatctttca catcatgaac
     1081 cgcgtaaagg tgcgtttgaa gttaacgatc tttatgccgt ggtttttgct gcattatcga
     1141 tcctgctgat ttatctgggc agtacaggaa tgtggccgct ccagtggatt ggcgcaggta
     1201 tgacggcgta tggattactc tattttatgg tgcacgacgg gctggtgcat caacgttggc
     1261 cattccgcta tattccacgc aagggctacc tcaaacggtt gtatatggcg caccgtatgc
     1321 atcacgccgt caggggcaaa gaaggttgtg tttcttttgg cttcctctat gcgccgcccc
     1381 tgtcaaaact tcaggcgacg ctccgggaaa gacatggcgc tagagcgggc gctgccagag
     1441 atgcgcaggg cggggaggat gagcccgcat ccgggaagta ataatactag agccggctta
     1501 tcggtcagtt tcacctgatt tacgtaaaaa cccgcttcgg cgggtttttg cttttggagg
     1561 ggcagaaaga tgaatgactg tccacgacgc tatacccaaa agaaa
//