LOCUS       BBa_K539558             2043 bp    DNA     linear       29-May-2024
DEFINITION  a RBS+lacI repressor+RNA thermometer+tetR+double terminator
ACCESSION   BBa_K539558
VERSION     BBa_K539558.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     RBS             1226..1231
                     /label=rbs
     misc_feature    1202..1207
                     /label=scar adaptation
     stem_loop       1180..1197
                     /label=stem_loop
     stem_loop       1203..1231
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1180..1231
                     /label=BBa_K115002
     CDS             1241..1857
                     /label=tetR
     misc_feature    1858..1891
                     /label=SsrA
     misc_feature    1898..1922
                     /label=Help:Barcodes
     CDS             1238..1922
                     /label=BBa_C0040
     terminator      1931..2043
                     /label=BBa_J61048
     misc_feature    1180..2043
                     /label=BBa_K332033
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagg gacaagcaat gcttgccttg
     1201 aatagtaact tttgaatagt gattcaggag gtactagatg tccagattag ataaaagtaa
     1261 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     1321 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     1381 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     1441 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     1501 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     1561 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     1621 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     1681 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     1741 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     1801 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     1861 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
     1921 actactagag ccggcttatc ggtcagtttc acctgattta cgtaaaaacc cgcttcggcg
     1981 ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacgcta tacccaaaag
     2041 aaa
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