LOCUS       BBa_K539654             2742 bp    DNA     linear       19-May-2024
DEFINITION  a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator
ACCESSION   BBa_K539654
VERSION     BBa_K539654.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..1870
                     /label=ilvD
     CDS             20..1870
                     /label=BBa_K539712
     misc_feature    1..1870
                     /label=BBa_K539625
     RBS             1925..1930
                     /label=rbs
     misc_feature    1901..1906
                     /label=scar adaptation
     stem_loop       1879..1896
                     /label=stem_loop
     stem_loop       1902..1930
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1879..1930
                     /label=BBa_K115002
     CDS             1940..2556
                     /label=tetR
     misc_feature    2557..2590
                     /label=SsrA
     misc_feature    2597..2621
                     /label=Help:Barcodes
     CDS             1937..2621
                     /label=BBa_C0040
     terminator      2630..2742
                     /label=BBa_J61048
     misc_feature    1879..2742
                     /label=BBa_K332033
ORIGIN
        1 tcacacagga aagtactaga tgcctaagta ccgttccgcc accaccactc atggtcgtaa
       61 tatggcgggt gctcgtgcgc tgtggcgcgc caccggaatg accgacgccg atttcggtaa
      121 gccgattatc gcggttgtga actcgttcac ccaatttgta ccgggtcacg tccatctgcg
      181 cgatctcggt aaactggtcg ccgaacaaat tgaagcggct ggcggcgttg ccaaagagtt
      241 caacaccatt gcggtggatg atgggattgc catgggccac ggggggatgc tttattcact
      301 gccatctcgc gaactgatcg ctgattccgt tgagtatatg gtcaacgccc actgcgccga
      361 cgccatggtc tgcatctcta actgcgacaa aatcaccccg gggatgctga tggcttccct
      421 gcgcctgaat attccggtga tctttgtttc cggcggcccg atggaggccg ggaaaaccaa
      481 actttccgat cagatcatca agctcgatct ggtcgatgcg atgatccagg gcgcagaccc
      541 gaaagtatct gactcccaga gcgatcaggt tgaacgttcc gcgtgtccga cctgcggttc
      601 ctgctccggg atgtttaccg ctaactcaat gaactgcctg accgaagcgc tgggcctgtc
      661 gcagccgggc aacggctcgc tgctggcaac ccacgccgac cgtaagcagc tgttccttaa
      721 tgctggtaaa cgcattgttg aattgaccaa acgttattac gagcaaaacg acgaaagtgc
      781 actgccgcgt aatatcgcca gtaaggcggc gtttgaaaac gccatgacgc tggatatcgc
      841 gatgggtgga tcgactaaca ccgtacttca cctgctggcg gcggcgcagg aagcggaaat
      901 cgacttcacc atgagtgata tcgataagct ttcccgcaag gttccacagc tgtgtaaagt
      961 tgcgccgagc acccagaaat accatatgga agatgttcac cgtgctggtg gtgttatcgg
     1021 tattctcggc gaactggatc gcgcggggtt actgaaccgt gatgtgaaaa acgtacttgg
     1081 cctgacgttg ccgcaaacgc tggaacaata cgacgttatg ctgacccagg atgacgcggt
     1141 aaaaaatatg ttccgcgcag gtccagcagg cattcgtacc acacaggcat tctcgcaaga
     1201 ttgccgttgg gatacgctgg acgacgatcg cgccaatggc tgtatccgct cgctggaaca
     1261 cgcctacagc aaagacggcg gcctggcggt gctctacggt aactttgcgg aaaacggctg
     1321 catcgtgaaa acggcaggcg tcgatgacag catcctcaaa ttcaccggcc cggcgaaagt
     1381 gtacgaaagc caggacgatg cggtagaagc gattctcggc ggtaaagttg tcgccggaga
     1441 tgtggtagta attcgctatg aaggcccgaa aggcggtccg gggatgcagg aaatgctcta
     1501 cccaaccagc ttcctgaaat caatgggtct cggcaaagcc tgtgcgctga tcaccgacgg
     1561 tcgtttctct ggtggcacct ctggtctttc catcggccac gtctcaccgg aagcggcaag
     1621 cggcggcagc attggcctga ttgaagatgg tgacctgatc gctatcgaca tcccgaaccg
     1681 tggcattcag ttacaggtaa gcgatgccga actggcggcg cgtcgtgaag cgcaggacgc
     1741 tcgaggtgac aaagcctgga cgccgaaaaa tcgtgaacgt caggtctcct ttgccctgcg
     1801 tgcttatgcc agcctggcaa ccagcgccga caaaggcgcg gtgcgcgata aatcgaaact
     1861 ggggggttaa tactagaggg acaagcaatg cttgccttga atagtaactt ttgaatagtg
     1921 attcaggagg tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga
     1981 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     2041 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     2101 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     2161 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     2221 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     2281 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     2341 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     2401 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     2461 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     2521 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc
     2581 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc cggcttatcg
     2641 gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt ttggaggggc
     2701 agaaagatga atgactgtcc acgacgctat acccaaaaga aa
//