LOCUS       BBa_K539655             2741 bp    DNA     linear       19-May-2024
DEFINITION  a RBS (B0034)+ilvD with RNA thermometer+tetR+terminator
ACCESSION   BBa_K539655
VERSION     BBa_K539655.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1869
                     /label=ilvD
     CDS             19..1869
                     /label=BBa_K539712
     misc_feature    1..1869
                     /label=BBa_K539626
     RBS             1924..1929
                     /label=rbs
     misc_feature    1900..1905
                     /label=scar adaptation
     stem_loop       1878..1895
                     /label=stem_loop
     stem_loop       1901..1929
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1878..1929
                     /label=BBa_K115002
     CDS             1939..2555
                     /label=tetR
     misc_feature    2556..2589
                     /label=SsrA
     misc_feature    2596..2620
                     /label=Help:Barcodes
     CDS             1936..2620
                     /label=BBa_C0040
     terminator      2629..2741
                     /label=BBa_J61048
     misc_feature    1878..2741
                     /label=BBa_K332033
ORIGIN
        1 aaagaggaga aatactagat gcctaagtac cgttccgcca ccaccactca tggtcgtaat
       61 atggcgggtg ctcgtgcgct gtggcgcgcc accggaatga ccgacgccga tttcggtaag
      121 ccgattatcg cggttgtgaa ctcgttcacc caatttgtac cgggtcacgt ccatctgcgc
      181 gatctcggta aactggtcgc cgaacaaatt gaagcggctg gcggcgttgc caaagagttc
      241 aacaccattg cggtggatga tgggattgcc atgggccacg gggggatgct ttattcactg
      301 ccatctcgcg aactgatcgc tgattccgtt gagtatatgg tcaacgccca ctgcgccgac
      361 gccatggtct gcatctctaa ctgcgacaaa atcaccccgg ggatgctgat ggcttccctg
      421 cgcctgaata ttccggtgat ctttgtttcc ggcggcccga tggaggccgg gaaaaccaaa
      481 ctttccgatc agatcatcaa gctcgatctg gtcgatgcga tgatccaggg cgcagacccg
      541 aaagtatctg actcccagag cgatcaggtt gaacgttccg cgtgtccgac ctgcggttcc
      601 tgctccggga tgtttaccgc taactcaatg aactgcctga ccgaagcgct gggcctgtcg
      661 cagccgggca acggctcgct gctggcaacc cacgccgacc gtaagcagct gttccttaat
      721 gctggtaaac gcattgttga attgaccaaa cgttattacg agcaaaacga cgaaagtgca
      781 ctgccgcgta atatcgccag taaggcggcg tttgaaaacg ccatgacgct ggatatcgcg
      841 atgggtggat cgactaacac cgtacttcac ctgctggcgg cggcgcagga agcggaaatc
      901 gacttcacca tgagtgatat cgataagctt tcccgcaagg ttccacagct gtgtaaagtt
      961 gcgccgagca cccagaaata ccatatggaa gatgttcacc gtgctggtgg tgttatcggt
     1021 attctcggcg aactggatcg cgcggggtta ctgaaccgtg atgtgaaaaa cgtacttggc
     1081 ctgacgttgc cgcaaacgct ggaacaatac gacgttatgc tgacccagga tgacgcggta
     1141 aaaaatatgt tccgcgcagg tccagcaggc attcgtacca cacaggcatt ctcgcaagat
     1201 tgccgttggg atacgctgga cgacgatcgc gccaatggct gtatccgctc gctggaacac
     1261 gcctacagca aagacggcgg cctggcggtg ctctacggta actttgcgga aaacggctgc
     1321 atcgtgaaaa cggcaggcgt cgatgacagc atcctcaaat tcaccggccc ggcgaaagtg
     1381 tacgaaagcc aggacgatgc ggtagaagcg attctcggcg gtaaagttgt cgccggagat
     1441 gtggtagtaa ttcgctatga aggcccgaaa ggcggtccgg ggatgcagga aatgctctac
     1501 ccaaccagct tcctgaaatc aatgggtctc ggcaaagcct gtgcgctgat caccgacggt
     1561 cgtttctctg gtggcacctc tggtctttcc atcggccacg tctcaccgga agcggcaagc
     1621 ggcggcagca ttggcctgat tgaagatggt gacctgatcg ctatcgacat cccgaaccgt
     1681 ggcattcagt tacaggtaag cgatgccgaa ctggcggcgc gtcgtgaagc gcaggacgct
     1741 cgaggtgaca aagcctggac gccgaaaaat cgtgaacgtc aggtctcctt tgccctgcgt
     1801 gcttatgcca gcctggcaac cagcgccgac aaaggcgcgg tgcgcgataa atcgaaactg
     1861 gggggttaat actagaggga caagcaatgc ttgccttgaa tagtaacttt tgaatagtga
     1921 ttcaggaggt actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
     1981 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     2041 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     2101 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     2161 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     2221 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     2281 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     2341 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     2401 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     2461 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     2521 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
     2581 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc ggcttatcgg
     2641 tcagtttcac ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca
     2701 gaaagatgaa tgactgtcca cgacgctata cccaaaagaa a
//