LOCUS BBa_K539673 4245 bp DNA linear 28-May-2024 DEFINITION a RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+ter minator ACCESSION BBa_K539673 VERSION BBa_K539673.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 CDS 20..1495 /label=ilvC coding sequence CDS 20..1495 /label=BBa_K539711 misc_feature 1..1495 /label=BBa_K539622 RBS 1504..1516 /label=RBS-3\Weak RBS 1510..1513 /label=RBS misc_feature 1504..1516 /label=BBa_B0032 RBS 1504..1516 /label=BBa_B0032 CDS 1523..3373 /label=ilvD CDS 1523..3373 /label=BBa_K539712 misc_feature 1504..3373 /label=BBa_K539625 misc_feature 1..3373 /label=BBa_K539641 RBS 3428..3433 /label=rbs misc_feature 3404..3409 /label=scar adaptation stem_loop 3382..3399 /label=stem_loop stem_loop 3405..3433 /label=predicted stem-loop extending to one position before start codon misc_feature 3382..3433 /label=BBa_K115002 CDS 3443..4059 /label=tetR misc_feature 4060..4093 /label=SsrA misc_feature 4100..4124 /label=Help:Barcodes CDS 3440..4124 /label=BBa_C0040 terminator 4133..4245 /label=BBa_J61048 misc_feature 3382..4245 /label=BBa_K332033 ORIGIN 1 tcacacagga aagtactaga tggctaacta cttcaataca ctgaatctgc gccagcagct 61 ggcacagctg ggcaaatgtc gctttatggg ccgcgatgaa tttgccgatg gcgcgagcta 121 ccttcagggt aaaaaagtag tcatcgtcgg ctgtggcgca cagggtctga accagggcct 181 gaacatgcgt gattctggtc tcgatatctc ctacgctctg cgtaaagaag cgattgccga 241 gaagcgcgcg tcctggcgta aagcgaccga aaatggtttt aaagtgggta cttacgaaga 301 actgatccca caggcggatc tggtgattaa cctgacgccg gacaagcagc actctgatgt 361 agtgcgcacc gtacagccac tgatgaaaga cggcgcggcg ctgggctact cgcacggttt 421 caacatcgtc gaagtgggcg agcagatccg taaagatatc accgtagtga tggttgcgcc 481 gaaatgccca ggcaccgaag tgcgtgaaga gtacaaacgt gggttcggcg taccgacgct 541 gattgccgtt cacccggaaa acgatccgaa aggcgaaggc atggcgattg ccaaagcctg 601 ggcggctgca accggtggtc accgtgcggg tgtgctggaa tcgtccttcg ttgcggaagt 661 gaaatctgat ctgatgggcg agcaaaccat cctgtgcggt atgttgcagg ctggctctct 721 gctgtgcttc gacaagctgg tggaagaagg taccgatcca gcatacgcag aaaaactgat 781 tcagttcggt tgggaaacca tcaccgaagc actgaaacag ggcggcatca ccctgatgat 841 ggaccgtctc tctaacccgg cgaaactgcg tgcttatgcg ctttctgaac agctgaaaga 901 gatcatggca cccctgttcc agaaacatat ggacgacatc atctccggcg aattttcttc 961 cggtatgatg gcggactggg ccaacgatga taagaaactg ctgacctggc gtgaagagac 1021 cggcaaaacc gcgtttgaaa ccgcgccgca gtatgaaggc aaaatcggcg agcaggagta 1081 cttcgataaa ggcgtactga tgattgcgat ggtgaaagcg ggcgttgaac tggcgttcga 1141 aaccatggtc gattccggca tcattgaaga gtctgcatat tatgaatcac tgcacgagct 1201 gccgctgatt gccaacacca tcgcccgtaa gcgtctgtac gaaatgaacg tggttatctc 1261 tgataccgct gagtacggta actatctgtt ctcttacgct tgtgtgccgt tgctgaaacc 1321 gtttatggca gagctgcaac cgggcgacct gggtaaagct attccggaag gcgcggtaga 1381 taacgggcaa ctgcgtgatg tgaacgaagc gattcgcagc catgcgattg agcaggtagg 1441 taagaaactg cgcggctata tgacagatat gaaacgtatt gctgttgcgg gttaatacta 1501 gagtcacaca ggaaagtact agatgcctaa gtaccgttcc gccaccacca ctcatggtcg 1561 taatatggcg ggtgctcgtg cgctgtggcg cgccaccgga atgaccgacg ccgatttcgg 1621 taagccgatt atcgcggttg tgaactcgtt cacccaattt gtaccgggtc acgtccatct 1681 gcgcgatctc ggtaaactgg tcgccgaaca aattgaagcg gctggcggcg ttgccaaaga 1741 gttcaacacc attgcggtgg atgatgggat tgccatgggc cacgggggga tgctttattc 1801 actgccatct cgcgaactga tcgctgattc cgttgagtat atggtcaacg cccactgcgc 1861 cgacgccatg gtctgcatct ctaactgcga caaaatcacc ccggggatgc tgatggcttc 1921 cctgcgcctg aatattccgg tgatctttgt ttccggcggc ccgatggagg ccgggaaaac 1981 caaactttcc gatcagatca tcaagctcga tctggtcgat gcgatgatcc agggcgcaga 2041 cccgaaagta tctgactccc agagcgatca ggttgaacgt tccgcgtgtc cgacctgcgg 2101 ttcctgctcc gggatgttta ccgctaactc aatgaactgc ctgaccgaag cgctgggcct 2161 gtcgcagccg ggcaacggct cgctgctggc aacccacgcc gaccgtaagc agctgttcct 2221 taatgctggt aaacgcattg ttgaattgac caaacgttat tacgagcaaa acgacgaaag 2281 tgcactgccg cgtaatatcg ccagtaaggc ggcgtttgaa aacgccatga cgctggatat 2341 cgcgatgggt ggatcgacta acaccgtact tcacctgctg gcggcggcgc aggaagcgga 2401 aatcgacttc accatgagtg atatcgataa gctttcccgc aaggttccac agctgtgtaa 2461 agttgcgccg agcacccaga aataccatat ggaagatgtt caccgtgctg gtggtgttat 2521 cggtattctc ggcgaactgg atcgcgcggg gttactgaac cgtgatgtga aaaacgtact 2581 tggcctgacg ttgccgcaaa cgctggaaca atacgacgtt atgctgaccc aggatgacgc 2641 ggtaaaaaat atgttccgcg caggtccagc aggcattcgt accacacagg cattctcgca 2701 agattgccgt tgggatacgc tggacgacga tcgcgccaat ggctgtatcc gctcgctgga 2761 acacgcctac agcaaagacg gcggcctggc ggtgctctac ggtaactttg cggaaaacgg 2821 ctgcatcgtg aaaacggcag gcgtcgatga cagcatcctc aaattcaccg gcccggcgaa 2881 agtgtacgaa agccaggacg atgcggtaga agcgattctc ggcggtaaag ttgtcgccgg 2941 agatgtggta gtaattcgct atgaaggccc gaaaggcggt ccggggatgc aggaaatgct 3001 ctacccaacc agcttcctga aatcaatggg tctcggcaaa gcctgtgcgc tgatcaccga 3061 cggtcgtttc tctggtggca cctctggtct ttccatcggc cacgtctcac cggaagcggc 3121 aagcggcggc agcattggcc tgattgaaga tggtgacctg atcgctatcg acatcccgaa 3181 ccgtggcatt cagttacagg taagcgatgc cgaactggcg gcgcgtcgtg aagcgcagga 3241 cgctcgaggt gacaaagcct ggacgccgaa aaatcgtgaa cgtcaggtct cctttgccct 3301 gcgtgcttat gccagcctgg caaccagcgc cgacaaaggc gcggtgcgcg ataaatcgaa 3361 actggggggt taatactaga gggacaagca atgcttgcct tgaatagtaa cttttgaata 3421 gtgattcagg aggtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 3481 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 3541 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 3601 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 3661 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 3721 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 3781 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 3841 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 3901 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 3961 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 4021 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 4081 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccggctta 4141 tcggtcagtt tcacctgatt tacgtaaaaa cccgcttcgg cgggtttttg cttttggagg 4201 ggcagaaaga tgaatgactg tccacgacgc tatacccaaa agaaa //