LOCUS       BBa_K539741             1848 bp    DNA     linear       19-May-2024
DEFINITION  TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate 
            decarboxylase)+Ter (BBa_J61048)
ACCESSION   BBa_K539741
VERSION     BBa_K539741.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1727
                     /label=kivd coding sequence
     CDS             81..1727
                     /label=BBa_K539714
     terminator      1736..1848
                     /label=BBa_J61048
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtatacag taggagatta cctattagac cgattacacg
      121 agttaggaat tgaagaaatt tttggagtcc ctggagacta taacttacaa tttttagatc
      181 aaattatttc ccgcaaggat atgaaatggg tcggaaatgc taatgaatta aatgcttcat
      241 atatggctga tggctatgct cgtactaaaa aagctgccgc atttcttaca acctttggag
      301 taggtgaatt gagtgcagtt aatggattag caggaagtta cgccgaaaat ttaccagtag
      361 tagaaatagt gggatcacct acatcaaaag ttcaaaatga aggaaaattt gttcatcata
      421 cgctggctga cggtgatttt aaacacttta tgaaaatgca cgaacctgtt acagcagctc
      481 gaactttact gacagcagaa aatgcaaccg ttgaaattga ccgagtactt tctgcactat
      541 taaaagaaag aaaacctgtc tatatcaact taccagttga tgttgctgct gcaaaagcag
      601 agaaaccctc actccctttg aaaaaagaaa actcaacttc aaatacaagt gaccaagaga
      661 tcttgaacaa aattcaagaa agcttgaaaa atgccaaaaa accaatcgtg attacaggac
      721 atgaaataat tagttttggc ttagaaaaaa cagtctctca atttatttca aagacaaaac
      781 tacctattac gacattaaac tttggaaaaa gttcagttga tgaagctctc ccttcatttt
      841 taggaatcta taatggtaaa ctctcagagc ctaatcttaa agtattcgtg gaatcagccg
      901 acttcatcct gatgcttgga gttaaactca cagactcttc aacaggagcc ttcactcatc
      961 atttaaatga aaataaaatg atttcactga atatagatga aggaaaaata tttaacgaaa
     1021 gcatccaaaa ttttgatttt gaatccctca tctcctctct cttagaccta agcgaaatag
     1081 aatacaaagg aaaatatatc gataaaaagc aagaagactt tgttccatca aatgcgcttt
     1141 tatcacaaga ccgcctatgg caagcagttg aaaacctaac tcaaagcaat gaaacaatcg
     1201 ttgctgaaca agggacatca ttctttggcg cttcatcaat tttcttaaaa ccaaagagtc
     1261 attttattgg tcaaccctta tggggatcaa ttggatatac attcccagca gcattaggaa
     1321 gccaaattgc agataaagaa agcagacacc ttttatttat tggtgatggt tcacttcaac
     1381 ttacggtgca agaattagga ttagcaatca gagaaaaaat taatccaatt tgctttatta
     1441 tcaataatga tggttataca gtcgaaagag aaattcatgg accaaatcaa agctacaatg
     1501 atattccaat gtggaattac tcaaaattac cagaatcatt tggagcaaca gaagaacgag
     1561 tagtctcgaa aatcgttaga actgaaaatg aatttgtgtc tgtcatgaaa gaagctcaag
     1621 cagatccaaa tagaatgtac tggattgagt taattttggc aaaagaagat gcaccaaaag
     1681 tactgaaaaa aatgggcaaa ctatttgctg aacaaaataa atcataatac tagagccggc
     1741 ttatcggtca gtttcacctg atttacgtaa aaacccgctt cggcgggttt ttgcttttgg
     1801 aggggcagaa agatgaatga ctgtccacga cgctataccc aaaagaaa
//