BBa_K546000

BBa_K546000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K546000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: M. Hesselman
Date created: 2011-09-13 11:00:00
Date modified: 2015-05-08 01:12:39

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.



Types
DnaRegion

Roles
Signalling

engineered_region

Sequences BBa_K546000_sequence (Version 1)

Description

The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.

Notes

The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.

Source

The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.

Access Instance Definition
public
public
BBa_F2621
BBa_K082014
BBa_F2621
BBa_K082014
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K546000/1