LOCUS BBa_K546546 3909 bp DNA linear 12-Jun-2024 DEFINITION The Synchroscillator: A Tunable Synchronized Oscillatory System ACCESSION BBa_K546546 VERSION BBa_K546546.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23101 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..63 /label=prefix misc_feature 235..235 /label=T misc_feature 553..553 /label=A CDS 62..811 /label=luxR misc_feature 62..817 /label=BBa_C0062 misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0062 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 misc_feature 44..979 /label=BBa_I0462 misc_feature 1..979 /label=BBa_K176025 misc_feature 988..1040 /label=R0062 promoter 1007..1012 /label=-35 promoter 1029..1034 /label=-10 misc_feature 1041..1059 /label=tetR misc_feature 1040..1040 /label=+1 misc_feature 988..1007 /label=luxR/HSL promoter 988..1059 /label=BBa_K176000 misc_feature 1..1059 /label=BBa_K176028 misc_feature 1072..1075 /label=conserved RBS 1068..1079 /label=BBa_B0034 misc_feature 1086..1088 /label=start misc_feature 1869..1874 /label=2 misc_feature 1836..1868 /label=LVA CDS 1086..1835 /label=aiiA misc_feature 1086..1874 /label=BBa_C0060 misc_feature 1875..1899 /label=Help:Barcodes CDS 1086..1874 /label=BBa_C0060 stem_loop 1919..1962 /label=stem_loop misc_feature 1908..1987 /label=BBa_B0010 terminator 1908..1987 /label=BBa_B0010 stem_loop 2003..2022 /label=T7 TE misc_feature 2029..2029 /label=stop polyA_site 2023..2036 /label=polya misc_feature 1996..2036 /label=BBa_B0012 terminator 1996..2036 /label=BBa_B0012 misc_feature 1068..2036 /label=BBa_I0460 promoter 2064..2118 /label=plac misc_feature 2064..2070 /label=-35 misc_feature 2086..2092 /label=-10 misc_feature 2071..2085 /label=O1 misc_feature 2093..2118 /label=O1 misc_feature 2045..2063 /label=luxR binding site promoter 2045..2118 /label=BBa_I751502 misc_feature 2131..2134 /label=conserved RBS 2127..2138 /label=BBa_B0034 promoter 2045..2138 /label=BBa_K182102 misc_feature 2724..2755 /label=LVA CDS 2145..2723 /label=luxI misc_feature 2145..2762 /label=BBa_C0061 misc_feature 2763..2787 /label=Help:Barcodes CDS 2145..2762 /label=BBa_C0061 stem_loop 2807..2850 /label=stem_loop misc_feature 2796..2875 /label=BBa_B0010 terminator 2796..2875 /label=BBa_B0010 stem_loop 2891..2910 /label=T7 TE misc_feature 2917..2917 /label=stop polyA_site 2911..2924 /label=polya misc_feature 2884..2924 /label=BBa_B0012 terminator 2884..2924 /label=BBa_B0012 terminator 2796..2924 /label=BBa_B0015 misc_feature 2933..2952 /label=LuxR/HSL promoter 2952..2957 /label=-35 promoter 2974..2979 /label=-10 misc_feature 2985..2985 /label=start misc_feature 2933..2987 /label=BBa_R0062 promoter 2933..2987 /label=BBa_R0062 misc_feature 3000..3003 /label=conserved RBS 2996..3007 /label=BBa_B0034 misc_feature 3014..3733 /label=BBa_E0040 misc_feature 3728..3766 /label=LVA misc_feature 3767..3772 /label=Double Stop misc_feature 3014..3016 /label=Start misc_feature 3014..3772 /label=BBa_J04031 stem_loop 3792..3835 /label=stem_loop misc_feature 3781..3860 /label=BBa_B0010 terminator 3781..3860 /label=BBa_B0010 stem_loop 3876..3895 /label=T7 TE misc_feature 3902..3902 /label=stop polyA_site 3896..3909 /label=polya misc_feature 3869..3909 /label=BBa_B0012 terminator 3869..3909 /label=BBa_B0012 misc_feature 2996..3909 /label=BBa_I763020 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat 1081 actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg ttggatcatt 1141 cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt tggtgttatc 1201 ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa agtgcagtta 1261 ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg aaaatgactg 1321 aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa gaccttcttt 1381 atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct tttataaata 1441 caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa gaatatttga 1501 aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat gaagtcgtac 1561 caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg ctattaattg 1621 agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg aaagagaatt 1681 ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct tcaattaaac 1741 gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat gatatagagc 1801 aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa aactacgctt 1861 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 1921 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1981 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2041 agagacctgt aggatcgtac aggtttactt gtgagcggat aacaatatag tgtgtggaat 2101 tgtgagcgga taacaattta ctagagaaag aggagaaata ctagatgact ataatgataa 2161 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 2221 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 2281 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 2341 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 2401 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 2461 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 2521 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 2581 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 2641 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 2701 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2761 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 2821 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2881 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 2941 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 3001 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 3061 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 3121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 3181 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 3241 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 3301 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 3361 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 3421 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 3481 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 3541 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 3601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 3661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 3721 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag 3781 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3841 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 3901 gcgtttata //