Aeq

BBa_K548000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K548000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peter Qiao
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:39

Humanized Aequorin



Types
DnaRegion

Roles
Signalling

engineered_region

Sequences BBa_K548000_sequence (Version 1)

Description

This is a human cell line optimized version of Aequorin, a bioluminescent protein found in various jellyfish and other marine organisms. The protein requires Ca2+ for catalytic activity. And oxidizes its substrate, Coelenterazine to emit blue light (λmax=469nm). Aequorin is NOT a fluorescent protein, it does not require an excitation light source to emit light. Transfection of the gene into 293T cells using lipofectamine has been confirmed. The part contains EcoRI and PstI restrictions sites, and is ligated to the iGEM pSB1C3 backbone. This backbone provides chloramphenicol resistance.

Notes

Original sequence had to be modified for increased expression in human cell lines.

Source

Synthesized from GeneArt, original sequence from NCBI, optimized using GeneArt codon optimization software.

igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K548000/1