BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K549038 1 BBa_K549038 Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z It originates from E.coli K12 MG 1655 gDNA. This is the promoter of rcnA, called prcnA. It has a binding site for the regulator RcnR, which originates in E. coli. In presence of Ni(II), Ni(II) binds to RcnR, RcnR binds to the promoter and activates transcription. Co(II) can also be a stimulus, but a weaker one. This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS. false false _717_ 0 6378 9 Not in stock false This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS. This BB can be used for measurement of Ni(II)-concentration. false Jara Radeck BBa_K549001 1 RcnA/RcnR Ni(II) dependent rcnA-promoter and GFP 2011-08-25T11:00:00Z 2015-05-08T01:12:39Z GFP is from E0040, prcnA is from genemic DNA (E. coli K12) E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed. false false _717_ 0 6347 9 It's complicated true Has to be used in E. coli, because Regulator RcnR (E. coli) is needed. false Jara Radeck component2137446 1 BBa_K549038 component2137448 1 BBa_E0040 annotation2137446 1 BBa_K549038 range2137446 1 1 213 annotation2137448 1 BBa_E0040 range2137448 1 220 939 BBa_K549001_sequence 1 acggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagcaaggaaacgcctggttcttcatccccagcgccatcttacttggtgcgtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K549038_sequence 1 acggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagcaaggaaacgcctggttcttcatccccagcgccatcttacttggtgcg BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z