BBa_K551000
1
BBa_K551000
FRT-CM-FRT
2011-09-18T11:00:00Z
2015-05-08T01:12:40Z
It comes from PCR of a registry part
This part is composed of two Flipase Recognition Targets (FRT) flanking the Chloramphenicol resistance gene.
false
false
_719_
0
4079
9
It's complicated
true
The initial part wasn't working, so we remade it from the intermediate
false
Laurence Van Melderen
component2136842
1
BBa_J61020
component2136844
1
BBa_P1004
component2136845
1
BBa_J61020
annotation2136844
1
BBa_P1004
range2136844
1
35
803
annotation2136842
1
BBa_J61020
range2136842
1
1
34
annotation2136845
1
BBa_J61020
range2136845
1
804
837
BBa_P1004
1
cmR
chloramphenicol resistance cassette
2006-02-03T12:00:00Z
2015-05-08T01:14:11Z
Released HQ 2013
same part as BBa_P1002 except in reverse orientation.
false
false
_41_6_
0
126
45
In stock
false
true
Reshma Shetty
annotation1897114
1
cmR
range1897114
1
107
769
BBa_J61020
1
FRT
[FRT]
2006-09-20T11:00:00Z
2015-08-31T02:02:59Z
<tt>
Extend ca1010F/R (73 bp, EcoRI/SpeI)<br>
Sub into pSB1A2-I13522 (EcoRI/SpeI)<br>
Product is pSB1A2-Bca1010<br>
----
ca1010F Forward (universal biobrick) EcoRI for FRT<br>
GGACTgaattcgcggccgcttctagag<br>
ca1010R Reverse SpeI oligo for FRT<br>
cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg<br>
</tt>
Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
false
false
_95_
0
483
95
It's complicated
false
N/A
false
John Anderson
BBa_K551000_sequence
1
gaagttcctatactttttagagaataggaacttcgttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataagaagttcctatactttttagagaataggaacttc
BBa_J61020_sequence
1
gaagttcctatactttttagagaataggaacttc
BBa_P1004_sequence
1
gttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z