LOCUS BBa_K557010 5701 bp DNA linear 01-Jun-2024 DEFINITION ColicinE7+Toggle-switch+Aptamer-CheZ+lysis ACCESSION BBa_K557010 VERSION BBa_K557010.1 FEATURES Location/Qualifiers RBS 117..124 /label=caeE7 RBS misc_feature 478..587 /label=caeE7 toxicity domain CDS 1862..1899 /label=Immunity (ceiE7) misc_feature 1..1899 /label=BBa_K117001 CDS 1908..2588 /label=mRFP CDS 4126..4845 /label=eGFP CDS 3468..4103 /label=CI434 repressor RBS 4110..4118 /label=rbs CDS 2614..3327 /label=lamda CI repressor RBS 2595..2607 /label=rbs RBS 3333..3339 /label=rbs promoter 3398..3446 /label=CI regulated promoter RBS 3453..3461 /label=rbs misc_feature 3346..3361 /label=CI434 OR1 promoter 3346..3397 /label=CI434 regulated promoter misc_feature 1908..4845 /label=BBa_K228003 misc_feature 4854..4899 /label=aptamer RBS 4900..4910 /label=rbs misc_feature 4911..4913 /label=start codon misc_feature 4854..5551 /label=BBa_K557001 misc_feature 1908..5551 /label=BBa_K557002 CDS 5558..5701 /label=BBa_K117000 misc_feature 1908..5701 /label=BBa_K557009 ORIGIN 1 aatatccctc cctgacttga cagagaaaat aatggcgagt actttttgat ctgtacataa 61 aaccagtggt tttatgtaca gtattaacta tgtaattaat tgttttaacg cttaaaagag 121 ggaattttta tgagcggtgg agatggacgt ggccacaaca gtggcgcaca taacacaggt 181 ggtaacatta atggcggccc tacggggctt ggtggaaatg gtggggcttc tgacggctcc 241 ggatggagtt cggaaaataa cccatggggt ggcggttccg gtagtggtgt tcactgggga 301 ggtggctccg gccatggcaa tggcgggggg aatagtaatt ccggtggtgg cagcaattca 361 tccgtagcag cacctatggc atttggtttt cctgctttgg ctgctcctgg tgccggaact 421 cttggtattt ccgtatctgg tgaggcttta tctgcggcaa tagcggatat ctttgcagcc 481 ctgaaaggtc cgtttaaatt cagcgcatgg ggtattgcgc tttacggcat tctgccatct 541 gaaatagcaa aagatgaccc gaatatgatg tcaaagattg tgacgtcatt accggcagaa 601 acagtgacga atgttcaggt cagtacactt ccgctggacc aggcaactgt cagcgtgacg 661 aaacgtgtga cagatgttgt gaaggacaca cgacagcata ttgccgtagt tgcaggtgtg 721 cctatgagtg tgcctgttgt gaatgccaaa ccaacacgta ctccaggtgt atttcacgca 781 tcatttcctg gtgtcccttc tctgacagtc agcactgtca aaggccttcc ggtgtcaaca 841 acccttcccc gtggtattac ggaggataaa ggccggactg ccgttcctgc gggatttacc 901 tttggtggtg gttcacatga agcggtgatt cgctttccga aagaaagtgg gcagaagccg 961 gtttatgtat cagtgacaga tgttcttacc ccagcacagg taaaacaacg tcaggatgag 1021 gaaaaacgcc tccagcagga atggaatgac gcacatccgg tggaagtggc tgaacgcaat 1081 tatgaacagg cccgcgcaga actgaatcag gctaataaag atgttgccag aaaccaggaa 1141 cgacaggcta aagctgttca ggtttataat tctcgtaaaa gtgaacttga tgcagcgaat 1201 aaaactcttg ctgatgcaaa ggctgaaata aaacaattcg agcgatttgc ccgagaacca 1261 atggctgctg gtcacagaat gtggcaaatg gcagggctta aggcccagcg ggcacagacg 1321 gatgtaaata ataagaaggc tgcatttgat gctgctgcaa aagaaaagtc agatgctgat 1381 gttgcattga gttctgcgtt ggagcgccgc aaacagaaag aaaataaaga aaaggacgct 1441 aaagccaaat tagataagga gagtaaacgg aataagccag ggaaggcaac aggtaaagga 1501 aaacctgtca ataataagtg gttaaataat gcaggtaaag acttaggttc tcctgttcca 1561 gatcgtatag ctaataaact acgtgataag gagtttaaaa gtttcgatga ttttcgtaag 1621 aaattctggg aagaagtgtc aaaagatcct gagttaagta aacaatttag taggaacaat 1681 aatgatcgaa tgaaggttgg aaaagcgccc aagactagaa cccaggatgt ttcagggaag 1741 agaacttcat tcgagcttca tcatgagaag ccgatcagcc aaaatggtgg tgtctatgat 1801 atggataaca tcagcgtggt aacacctaaa aggcatattg atattcaccg aggtaaataa 1861 tatggaactg aaaaatagta ttagtgatta cacagaggct actagagtta ttaagcaccg 1921 gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg 1981 gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta 2041 gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc 2101 attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc 2161 caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt 2221 ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca 2281 ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc 2341 aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc 2401 ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg 2461 gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg 2521 ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag 2581 gaagccatct agtatttctc ctctttcact agatcagcca aacgtctctt caggccactg 2641 actagcgata actttcccca caacggaaca actctcattg catgggatca ttgggtactg 2701 tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa 2761 ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc 2821 aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga 2881 attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca 2941 tctctccgca tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac 3001 atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc 3061 atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag 3121 aatttttgca agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc 3181 accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag 3241 ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg 3301 tgttaatggt ttcttttttg tgctcatttt tttcctccta ctagaaacaa actttcttgt 3361 acatacgtta aatctaacaa actttcttgt aaatatctaa caccgtgcgt gttgactatt 3421 ttacctctgg cggtgataat ggttgcacta gatactaagg aggttgtatg agtatttctt 3481 ccagggtaaa aagcaaaaga atccagcttg gacttaacca ggctgaactt gctcaaaagg 3541 tggggactac ccagcagtct atagagcagc tcgaaaacgg taaaactaag cgaccacgct 3601 ttttaccaga acttgcgtca gctcttggcg taagtgttga ctggctgctc aatggcacct 3661 ctgattcgaa tgttagattt gttgggcacg ttgagcccaa agggaaatat ccattgatta 3721 gcatggttag agctggttcg tggtgtgaag cttgtgaacc ctacgatatc aaggacattg 3781 atgaatggta tgacagtgac gttaacttat taggcaatgg attctggctg aaggttgaag 3841 gtgattccat gacctcacct gtaggtcaaa gcatccctga aggtcatatg gtgttagtag 3901 atactggacg ggagccagtg aatggaagcc ttgttgtagc caaactgact gacgcgaacg 3961 aagcaacatt caagaaactg gtcatagatg gcggtcagaa gtacctgaaa ggcctgaatc 4021 cttcatggcc tatgactcct atcaacggaa actgcaagat tatcggtgtt gtcgtggaag 4081 cgagggtaaa attcgtataa taaactagaa gaggagaaat taagcatggt gagcaagggc 4141 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 4201 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 4261 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 4321 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 4381 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 4441 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 4501 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 4561 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 4621 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 4681 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 4741 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 4801 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatacta gagggtgata 4861 ccagcatcgt cttgatgccc ttggcagcac cccgctgcaa gacaacaaga tgcaaccatc 4921 aatcaaacct gctgacgagc attcagctgg cgatatcatt gcgcgcatcg gcagcctgac 4981 gcgtatgctg cgcgacagtt tgcgggaact ggggctggat caggccattg ccgaagcggc 5041 ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc 5101 tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc aaatggagaa 5161 atcagcaaaa gcgttaaccc aacgttggga tgactggttt gccgatccga ttgaccttgc 5221 cgacgcccgt gaactggtaa cagatacacg acaatttctg gcagatgtac ccgcgcatac 5281 cagctttact aacgcgcaac tgctggaaat catgatggcg caggattttc aggatctcac 5341 cgggcaggtc attaagcgga tgatggatgt cattcaggag atcgaacgcc agttgctgat 5401 ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa accagagttt 5461 gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc aggatcaggt 5521 ggacgatttg ttggatagac ttggattttg atactagatg aaaaaaataa cagggattat 5581 tttattgctt cttgcagcca ttattcttgc tgcatgtcag gcaaactata tccgtgatgt 5641 tcagggcggg acagtatcac cgtcgtcaac tgctgaactg accggagtgg aaacgcagta 5701 a //