Pgad

BBa_K559011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K559011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jacky, Fong Chuen, Loo

Date created: 2011-10-27 11:00:00

Date modified: 2015-05-08 01:12:41

Pgad - Intracellular Chloride-Sensing Cassette

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K559011_sequence (Version 1)

Description

Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment.
In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.

Notes

Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.

Source

The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
gadC Start	1249,1251	feature/start, start_codon
PgadR	141,158	promoter, feature/promoter
gadR	275,1105	CDS, feature/protein
Pgad	1163,1181	feature/promoter, promoter
RBS_gadC	1237,1245	feature/rbs, ribosome_entry_site
RBS_gadR	264,269	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K559011/1

Member of these Collections

• //classic/regulatory/other
• //classic/signalling/receiver
• //function/cellsignalling
• iGEM Parts Registry