LOCUS       BBa_K561004             3813 bp    DNA     linear       20-May-2024
DEFINITION  PfdhF+cex+cenA+tetR
ACCESSION   BBa_K561004
VERSION     BBa_K561004.1  
FEATURES             Location/Qualifiers
     promoter        1..99
                     /label=BBa_K387003
     misc_feature    112..115
                     /label=conserved
     RBS             108..119
                     /label=BBa_B0034
     CDS             126..1583
                     /label=cex coding sequence
     misc_feature    906..911
                     /label=PstI site removed
     CDS             126..1586
                     /label=BBa_K118022
     misc_feature    1599..1602
                     /label=conserved
     RBS             1595..1606
                     /label=BBa_B0034
     CDS             1613..2962
                     /label=cenA coding sequence
     CDS             1613..2965
                     /label=BBa_K118023
     misc_feature    2978..2981
                     /label=conserved
     RBS             2974..2985
                     /label=BBa_B0034
     CDS             2995..3611
                     /label=tetR
     misc_feature    3612..3645
                     /label=SsrA
     misc_feature    3652..3676
                     /label=Help:Barcodes
     CDS             2992..3651
                     /label=BBa_C0040
     stem_loop       3696..3739
                     /label=stem_loop
     misc_feature    3685..3764
                     /label=BBa_B0010
     terminator      3685..3764
                     /label=BBa_B0010
     stem_loop       3780..3799
                     /label=T7 TE
     misc_feature    3806..3806
                     /label=stop
     polyA_site      3800..3813
                     /label=polya
     misc_feature    3773..3813
                     /label=BBa_B0012
     terminator      3773..3813
                     /label=BBa_B0012
     misc_feature    2974..3813
                     /label=BBa_P0440
ORIGIN
        1 tgaataaata gtgcccgtaa tatcagggaa tgaccccaca taaaatgtgg cataaaagat
       61 gcatactgta gtcgagagcg cgtatgcgtg atttgattat actagagaaa gaggagaaat
      121 actagatgcc taggaccacg cccgcacccg gccacccggc ccgcggcgcc cgcaccgctc
      181 tgcgcacgac gctcgccgcc gcggcggcga cgctcgtcgt cggcgccacg gtcgtgctgc
      241 ccgcccaggc cgcgaccacg ctcaaggagg ccgccgacgg cgccggccgg gacttcggct
      301 tcgcgctcga ccccaaccgg ctctcggagg cgcagtacaa ggcgatcgcc gacagcgagt
      361 tcaacctcgt cgtcgccgag aacgcgatga agtgggacgc caccgagccc tcgcagaaca
      421 gcttctcctt cggcgcgggc gaccgcgtcg cgagctacgc cgccgacacc ggcaaggagc
      481 tgtacggcca cacgctcgta tggcactcgc agctgcccga ctgggcgaag aacctcaacg
      541 gctccgcgtt cgagagcgcg atggtcaacc acgtgacgaa ggtcgccgac cacttcgagg
      601 gcaaggtcgc gtcgtgggac gtcgtcaacg aggcgttcgc cgacggcggc ggccgccggc
      661 aggactcggc gttccagcag aagctcggca acggctacat cgagaccgcg ttccgggcgg
      721 cacgtgcggc ggacccgacc gccaagctgt gcatcaacga ctacaacgtc gagggcatca
      781 acgcgaagag caactcgctc tacgacctcg tcaaggactt caaggcgcgc ggcgtcccgc
      841 tcgactgcgt cgggttccag tcgcacctca tcgtcggcca ggtgccgggc gacttccggc
      901 agaacctgca acggttcgcg gacctgggcg tggacgtgcg catcaccgag ctcgacatcc
      961 gcatgcggac gccctccgac gcgaccaagc tcgcgaccca ggcggccgac tacaagaagg
     1021 tcgtgcaggc ctgcatgcag gtgacccgct gccagggcgt gaccgtctgg ggcatcaccg
     1081 acaagtactc gtgggtgccg gacgtcttcc cgggcgaggg ggccgcgctg gtgtgggacg
     1141 cgagctacgc caagaagccg gcctacgccg ccgtgatgga ggccttcggc gcgagcccga
     1201 cgccgacgcc caccacgccg accccgacgc ccacgacgcc gacgccgacc ccgacgtccg
     1261 gtccggccgg gtgccaggtg ctgtggggcg tcaaccagtg gaacaccggc ttcaccgcga
     1321 acgtcaccgt gaagaacacg tcctccgctc cggtcgacgg ctggacgctc acgttcagct
     1381 tcccgtccgg ccagcaggtc acccaggcgt ggagctcgac ggtcacgcag tccggctcgg
     1441 ccgtgacggt ccgcaacgcc ccgtggaacg gctcgatccc ggcgggcggc accgcgcagt
     1501 tcggcttcaa cggctcgcac acgggcacca acgccgcgcc gacggcgttc tcgctcaacg
     1561 gcacgccctg cacggtcggc taataatact agagaaagag gagaaatact agatgtccac
     1621 ccgcagaacc gccgcagcgc tgctggcggc cgcggccgtc gccgtcggcg gtctgaccgc
     1681 cctcaccacc accgccgcgc aggcggctcc cggctgccgc gtcgactacg ccgtcaccaa
     1741 ccagtggccc ggcggcttcg gcgccaacgt cacgatcacc aacctcggcg accccgtctc
     1801 gtcgtggaag ctcgactgga cctacaccgc aggccagcgg atccagcagc tgtggaacgg
     1861 caccgcgtcg accaacggcg gccaggtctc cgtcaccagc ctgccctgga acggcagcat
     1921 cccgaccggc ggcacggcgt cgttcgggtt caacggctcg tgggccgggt ccaacccgac
     1981 gccggcgtcg ttctcgctca acggcaccac ctgcacgggc accgtgccga cgaccagccc
     2041 cacgccgacc ccgacgccga cgacccccac gccgacgccg accccgaccc ccacccccac
     2101 gccgacggtc acgccgcagc cgaccagcgg cttctacgtc gacccgacga cgcagggcta
     2161 ccgcgcgtgg caggccgcgt ccggcacgga caaggcgctg ctcgagaaga tcgcgctcac
     2221 cccgcaggcg tactgggtcg gcaactgggc cgacgcgtcg cacgcgcagg ccgaggtcgc
     2281 cgactacacc ggccgcgccg tcgcggccgg gaagacgccg atgctcgtcg tctacgcgat
     2341 cccgggccgc gactgcggct cgcactccgg cggtggtgtg tccgagtccg agtacgcgcg
     2401 ctgggtcgac accgtcgcgc agggcatcaa gggcaacccg atcgtgatcc tcgagcccga
     2461 cgcgctcgcg cagctcggcg actgctccgg ccagggtgac cgcgtcggct tcctcaagta
     2521 cgccgccaag tcgctcaccc tcaagggcgc gcgcgtctac atcgacgcgg gccacgcgaa
     2581 gtggctgtcg gtcgacacgc cggtgaaccg cctcaaccag gtcggcttcg agtacgcggt
     2641 gggcttcgcg ctcaacacgt cgaactacca gacgacggcg gacagcaagg cgtacggcca
     2701 gcagatctcg cagcggctgg gcggcaagaa gttcgtcatc gacacctcgc gcaacggcaa
     2761 cggctcgaac ggcgagtggt gcaacccgcg cggccgcgcg ctcggcgaac gcccggtcgc
     2821 ggtgaacgac ggctccggcc tggacgcgct cctgtgggtc aagctgcccg gcgagtccga
     2881 cggcgcgtgc aacggcggcc cggccgccgg ccagtggtgg caggagatcg ccctggagat
     2941 ggcgcgcaac gccaggtggt aataatacta gagaaagagg agaaatacta gatgtccaga
     3001 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
     3061 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
     3121 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
     3181 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
     3241 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
     3301 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
     3361 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
     3421 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
     3481 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
     3541 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
     3601 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag
     3661 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     3721 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     3781 gctcaccttc gggtgggcct ttctgcgttt ata
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