LOCUS       BBa_K561005             3290 bp    DNA     linear       17-May-2024
DEFINITION  Pvgb+bglX+tetR
ACCESSION   BBa_K561005
VERSION     BBa_K561005.1  
FEATURES             Location/Qualifiers
     promoter        1..135
                     /label=vgb promoter
     promoter        1..135
                     /label=BBa_K561001
     RBS             144..156
                     /label=RBS-3\Weak
     RBS             150..153
                     /label=RBS
     misc_feature    144..156
                     /label=BBa_B0032
     RBS             144..156
                     /label=BBa_B0032
     CDS             163..2439
                     /label=bglX coding sequence
     CDS             163..2442
                     /label=BBa_K118028
     misc_feature    2455..2458
                     /label=conserved
     RBS             2451..2462
                     /label=BBa_B0034
     CDS             2472..3088
                     /label=tetR
     misc_feature    3089..3122
                     /label=SsrA
     misc_feature    3129..3153
                     /label=Help:Barcodes
     CDS             2469..3128
                     /label=BBa_C0040
     stem_loop       3173..3216
                     /label=stem_loop
     misc_feature    3162..3241
                     /label=BBa_B0010
     terminator      3162..3241
                     /label=BBa_B0010
     stem_loop       3257..3276
                     /label=T7 TE
     misc_feature    3283..3283
                     /label=stop
     polyA_site      3277..3290
                     /label=polya
     misc_feature    3250..3290
                     /label=BBa_B0012
     terminator      3250..3290
                     /label=BBa_B0012
     misc_feature    2451..3290
                     /label=BBa_P0440
ORIGIN
        1 acaggacgct ggggttaaaa gtatttgagt tttgatgtgg attaagtttt aagaggcaat
       61 aaagattata ataagtgctg ctacaccata ctgatgtatg gcaaaaccat aataatgaac
      121 ttaaggaaga ccctctacta gagtcacaca ggaaagtact agatgaaaaa aataaccgta
      181 ttgatttcca tctggctcag tgcagccgct tttttctctt gtgagaaaaa aacagaagcg
      241 gggagtaaac ctcaggcctt tgataaagaa gtacaggatc tgttaaaaaa tatgtcgctg
      301 gaagaaaaag ccggacagat gacgcagatc gatatccgta atttattgaa caacggatat
      361 ggaaataccg atgaaaaatt agataccgcc agattaaaag aagccattca aacctatcat
      421 gtcggttcta tattaaattg cattcaggca tacacacctg aaaaatgggt tgaattgatt
      481 tctcagatcc agaacgaagc attgcaatca cctaataaaa ttccggtttt atacggaaca
      541 gatgccatgc atggtgttgg ctttattaaa gatgccgtat tgtttccgca taacataggt
      601 atggctgcat cccgcaacga tcaattggtt tcgcaggcag ctcaggtaac gtcaacagaa
      661 gcgcgttccg ttggccttac ctggaatttt gctccggtgc ttgacgtagg cagagaacct
      721 tactggtcgc gctttgaaga aacctttggt gaagatgtat acataacaac acaaatgggt
      781 tcagcagccg tacaaatgat ggaaggcagt gacctgacgt ctaaaacaaa catagcttcc
      841 tgcctgaaac actttattgg atattcagca cccaaaaatg gtatagaccg cacacaatca
      901 cacattcctg aaattgtgct gcgcgaatat tatctgcccc cgttcagaga agcgatcaat
      961 aaaggcgctt catccattat gatcaactct gctgagatca atggcattcc gtgtcacggc
     1021 aacaaatggc tgttaacaga tctgctccgt acagagcttg gatttaccgg aatggttgta
     1081 tcggattggg aagatgtgat ccgtttgcac acctggcata aagtagctgc tacgccgaaa
     1141 gaagccgtaa tgatggccgt taacgcaggt gtggatatga gcatggtgcc gaatgattac
     1201 tcgtttccga aatatctggt agaattggtg aaagaaggaa aagtttctat ggcgcgcatc
     1261 gacgaagcgg tgggacgtat tctgaccctg aaaatcaaac tgggcttgat gaaaaatcca
     1321 ttgccatcca ttgcagatgt tggtgtagtg ggttctgatg cacatcaaca gatcgcacta
     1381 aatgcagcgc gtgaatcaat tacattattg aagaacgata aaaatattct gcctcttgca
     1441 aaagataaaa aaatactatt ggttggtccg gccgctaaca gtttatctgc gctgcacagt
     1501 tcatggtcat acacatggca gggaagcaat gaatcgctgt atcctgaaac aactaaaaca
     1561 atccgtgagg cattagaagc gagcggtaat aaagctaaca tcagaacgaa tgcgactaca
     1621 ggatttgatg atgcagcaaa ttatgatgtt tcctttattc aaaaaaatac agcaggggtg
     1681 gatgtaatta ttgtgtgtgt aggtgaagct gcctatgccg aacagcctgg agtaatcaag
     1741 gatctcaatc tgccggaagc gcagaagcaa ttgattgttg ctgcaaagaa aacaggcaaa
     1801 cctgtcattg tatgcctggt agagggcagg ccgcgtttgt ttccggaaga agaagcactt
     1861 gcagatgctg taataatgtg ctaccgtccg ggcagcaaag gtgcagacgc atttgcagag
     1921 attctatacg gtgacatcaa tcccagcggt aagcttccgt tcacctatcc gagatacgac
     1981 ggagatatta ctacatacga ttataaattt aaggaaaccg agcagcagtt aaaaccgggc
     2041 gtgagtgaat ttgttgcgtt caatccgcaa tggccattcg gacatggctt gagctacaca
     2101 acgtttgcat attcaaacct taacgtgaac aaaagtaatt ttacgaagaa cgatagtgtg
     2161 ctggtaacgg tagacataag caatacagga gcacgtacag gtaagatagc cgtagagtta
     2221 tattccagag accacttcgc atcgattacg ccttctgaaa gacgcttacg taaatacaca
     2281 aagattgaac tgaaagcagg agagaagcaa accgtttcgt ttacgatcaa agcagcggat
     2341 ctgcaatttg taaataaaga tctgaaaaca gtaaccgaag caggcgcttt tgatctgatg
     2401 atcggaaatc tccagacaga aatatatttt aatgagtaat aatactagag aaagaggaga
     2461 aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     2521 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     2581 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     2641 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     2701 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     2761 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     2821 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     2881 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     2941 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     3001 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     3061 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag
     3121 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg
     3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//