Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduD20 Targeting Tag
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_K562001_sequence (Version 1) |
Description
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the first 20 amino acid residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2. This is a targeting sequence for the Salmonella Pdu bacterial microcompartment (BMC). This 20 residue targeting sequence has been tested by fusion to GFP and it does work. The construct is coloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20 in the Sargent Laboratory, Dundee, UK.Notes
The fragment carries an XbaI site at the extreme 3' end. Thus, to clone your gene of interest in-frame with this targeting sequence all you need do is place the 6-nucleotide XbaI site immediately upstream of your ATG initiation codon.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562000 synthetic RBS PduD20 Tag | 1,103 107,112 119,178 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs tag feature/tag |