BBa_K562008 1 Salty_PduA Salty_PduABTUN BMC Components 2011-09-15T11:00:00Z 2015-05-08T01:12:41Z Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence. This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA, PduB, PduT, and PduU proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562007) AND protein PduN. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB, PduU and PduN gene products carry C-terminal 6-His affinity/epitope tags. Production of the PduB, PduU and PduN proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT, PduU and PduN have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTUN in the Sargent Laboratory, Dundee, UK. false false _730_ 0 8083 9 In stock false Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3'end. false Frank Sargent annotation2129502 1 pduU gene range2129502 1 1806 2153 annotation2129495 1 BBa_K562007 range2129495 1 1 2180 annotation2129496 1 synthetic RBS range2129496 1 107 112 annotation2129510 1 pduN gene range2129510 1 2196 2468 annotation2129503 1 Hexa-Histidine Tag range2129503 1 2154 2171 annotation2129512 1 Hexa-Histidine Tag range2129512 1 2469 2486 annotation2129498 1 pduB gene range2129498 1 400 1209 annotation2129500 1 synthetic RBS range2129500 1 1240 1245 annotation2129497 1 pduA gene range2129497 1 119 403 annotation2129507 1 synthetic RBS range2129507 1 2184 2189 annotation2129499 1 Hexa-Histidine Tag range2129499 1 1210 1227 annotation2129501 1 pduT gene range2129501 1 1252 1806 BBa_K562008_sequence 1 tgtcggttggcgcaaaacacgctgattttttcatcgctcaaggcgggccgtgtaacgtataatgcggctttgtttaatcatcatctaccacagaggaggatcacacagaggaacaggtatgcaacaagaagcactaggaatggtagaaaccaaaggcttaaccgcagccatagaggccgctgatgcaatggttaagtcagccaatgtgatgttagtgggctatgaaaagattggctccgggctggtaaccgtcatcgtgcgcggcgatgttggcgcggtcaaagcggccaccgatgcaggtgccgcagccgcacgcaacgtgggtgaagtgaaagccgtacacgtcatcccacgccctcacaccgatgtagaaaaaatcttaccgaagggaattagccaatgagcagcaatgagctggtggaacagatcatggcgcaggtgattgcccgtgtggcaacgccggaacaacaggccatccctggtcaaccccaacctatacgagagacggctatggcagaaaaaagctgtagtttaacggaatttgtcgggaccgccattggcgataccctgggcctggttatcgccaacgtcgataccgccctgctggacgcgatgaaacttgagaagcgctatcgttccatcggcattctcggcgcccgcaccggcgcaggcccgcacattatggcggcggacgaagcggtgaaagccaccaacaccgaagtggtcagcattgagctgccgcgcgataccaaaggcggcgcgggccacggttcgctgattattttaggcggtaacgacgtttccgacgtcaagcgcggtatcgaagtcgcgctgaaagagctggaccgcaccttcggtgatgtttacggcaacgaagccggacatatcgagctgcaatacaccgctcgcgccagctatgcgctggaaaaagcgttcggcgcgccgattggccgcgcctgcggcattatcgtcggcgctccggcttccgtcggcgtactgatggccgataccgcgctgaagtcagccaacgttgaagtcgtggcgtacagctctccggcgcacggcaccagctttagtaacgaagctattctggtgatttccggcgactccggcgcggtccgtcaggcggtgacctccgcccgcgaaatcggcaaaaccgtccttgcgaccctcggttctgaaccgaaaaacgatcgtccgtcctacatccatcaccatcaccatcactaaggatcacacagaggaacaggtatgtctcaggctataggaattttagaactcaccagcatcgccaaaggcatggagcttggtgatgccatgctgaaaagcgccaacgtcgatctactggtcagcaagaccatctgtccggggaagtttttactgatgctgggcggcgatatcggcgctatccagcaggccattgaaaccggtacgtcgcaggccggcgagatgctcgtcgacagcctggtgctggcgaatattcatcccagcgtacttccggccatcagcggcctgaacagcgtcgataagcgccaggcggtaggtatcgtcgaaacctggagcgtggcggcctgcattagcgccgcagaccgggcggtgaaaggctcaaacgtcaccctggtgcgggtgcatatggccttcggtatcggcggtaaatgctacatggtggtggcgggcgacgtttctgacgtcaataacgccgtgacggttgccagcgaaagcgcgggcgagaaagggttgttggtttaccgttcggtgatcccacgcccgcatgaagccatgtggcgacagatggtggaggggtaatggaaagacaaccgacaacggatcgcatgattcaggaatacgtgccggggaaacaggtcactctcgcgcacctgattgctaatccagggaaagatctctttaagaagctgggcctgcaagatgcagtgtccgccattggcatccttaccatcacgccgagcgaagcatcgattatcgcctgcgatatcgccaccaaatccggcgcggtggaaattggttttctcgatcgctttaccggtgccgtggtgctgaccggcgacgtttctgccgtcgagtatgccctcaagcaggtaacgcgcacgctgggcgaaatgatgcagttcaccacttgctcgatcacccggacgcatcaccatcaccatcactaaggatcacacagaggaacaggtatgcatctggcacgagtcacgggcgcggttgtctccacgcaaaaatcaccttctttgattgggaaaaagctgctgctggtgcgtcgggtcagcgccgatggcgaactccccgcctcgcccacctccggcgatgaagtggccgtggactccgtcggcgcgggcgtcggcgaactggttttgctcagcggcggctccagcgccaggcacgttttttccgggccaaatgaggccattgacctcgccgttgtcggcattgtagatacgctttcgtgtcatcaccatcaccatcactaaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z