BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K566013
1
RFP opt
RFP optimized for E. coli (+LVA)
2011-09-25T11:00:00Z
2015-05-08T01:12:43Z
Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.
RFP optimized with preferential codons for better expression into E. coli.
Includes LVA tag.
false
false
_734_
0
8650
9
Not in stock
false
Sequence was design considering use of preferential codons, as well, a complete search looking for illegal sites for classic BioBricks was performed, they were found and deleted.
false
Daniel Rodriguez
annotation2142584
1
LVA
range2142584
1
709
717
annotation2142583
1
mCherry (RFP)
range2142583
1
1
708
BBa_I12006
1
Prm +
Modified lamdba Prm promoter (repressed by 434 cI)
2004-07-13T11:00:00Z
2015-08-31T04:07:31Z
Bushman(1993), Shih & Gussin (1983)
Released HQ 2013
Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
false
false
_3_
0
147
7
In stock
false
The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
true
mcnamara
annotation786500
1
OR1 lambda
range786500
1
9
25
annotation786518
1
OR2 lambda
range786518
1
33
49
annotation786365
1
OR1 434
range786365
1
56
69
annotation837284
1
-35
range837284
1
48
53
annotation837228
1
-10
range837228
1
71
76
BBa_K566017
1
BBa_K566017
pRM434-RFP
2011-09-25T11:00:00Z
2015-05-08T01:12:43Z
Synthesized DNA.
Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch.
false
false
_734_
0
8650
9
It's complicated
true
Search for illegal sites was performed, sites were localized and deleted.
false
Daniel Rodriguez
component2142702
1
BBa_I12006
component2142704
1
BBa_B0030
component2142708
1
BBa_K566013
annotation2142708
1
BBa_K566013
range2142708
1
112
834
annotation2142704
1
BBa_B0030
range2142704
1
91
105
annotation2142702
1
BBa_I12006
range2142702
1
1
82
BBa_K566017_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagattaaagaggagaaatactagatggttagcaaaggtgaagaggataacatggccatcatcaaagagttcatgcgctttaaagttcacatggaaggtagcgttaatggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttatgtatggtagcaaagcctatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctataaagttaaactgcgtggcaccaattttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcagcgaacgtatgtatccggaagatggcgcactgaaaggtgaaattaaacagcgcctgaaactgaaagatggcggtcattatgatgcagaagttaaaaccacctataaagccaaaaaaccggttcagctgcctggtgcatataacgttaacattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtggtatggatgaactgtataaactggttgcctgataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K566013_sequence
1
atggttagcaaaggtgaagaggataacatggccatcatcaaagagttcatgcgctttaaagttcacatggaaggtagcgttaatggccacgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttatgtatggtagcaaagcctatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctataaagttaaactgcgtggcaccaattttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcagcgaacgtatgtatccggaagatggcgcactgaaaggtgaaattaaacagcgcctgaaactgaaagatggcggtcattatgatgcagaagttaaaaccacctataaagccaaaaaaccggttcagctgcctggtgcatataacgttaacattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtggtatggatgaactgtataaactggttgcctgataa
BBa_I12006_sequence
1
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z