LOCUS       BBa_K574004              797 bp    DNA     linear       07-May-2024
DEFINITION  3OC12HSL regulated by TetR
ACCESSION   BBa_K574004
VERSION     BBa_K574004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..686
                     /label=LasI
     misc_feature    687..719
                     /label=LVA
     misc_feature    726..750
                     /label=Help:Barcodes
     CDS             84..750
                     /label=BBa_C0078
     misc_feature    759..797
                     /label=B1006
     stem_loop       768..789
                     /label=modified thr terminator
     polyA_site      790..797
                     /label=PolyA
     polyA_site      759..767
                     /label=PolyA
     terminator      759..797
                     /label=BBa_B1006
     misc_feature    63..797
                     /label=BBa_K081016
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
      121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
      181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
      241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
      301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
      361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
      421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
      481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
      541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
      601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
      661 tggttgaaca gcgtctggct gtttccgctg ctaacgacga aaactacgct ctggttgctt
      721 aataactctg atagtgctag tgtagatctc tactagagaa aaaaaaaccc cgcccctgac
      781 agggcggggt ttttttt
//