LOCUS       BBa_K574005             1712 bp    DNA     linear       02-May-2024
DEFINITION  3OC12HSL and YFP regulated by pBad
ACCESSION   BBa_K574005
VERSION     BBa_K574005.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     CDS             160..762
                     /label=LasI
     misc_feature    763..795
                     /label=LVA
     misc_feature    802..826
                     /label=Help:Barcodes
     CDS             160..826
                     /label=BBa_C0078
     misc_feature    139..826
                     /label=BBa_K081009
     misc_feature    839..842
                     /label=conserved
     RBS             835..846
                     /label=BBa_B0034
     CDS             853..1575
                     /label=BBa_E0030
     stem_loop       1595..1638
                     /label=stem_loop
     misc_feature    1584..1663
                     /label=BBa_B0010
     terminator      1584..1663
                     /label=BBa_B0010
     stem_loop       1679..1698
                     /label=T7 TE
     misc_feature    1705..1705
                     /label=stop
     polyA_site      1699..1712
                     /label=polya
     misc_feature    1672..1712
                     /label=BBa_B0012
     terminator      1672..1712
                     /label=BBa_B0012
     misc_feature    835..1712
                     /label=BBa_E0430
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tgatcgttca gatcggtcgt
      181 cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc
      241 aaagaacgta aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac
      301 gctctgtccc cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt
      361 tgctggcgta tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg
      421 ctgcacggta aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc
      481 aactccggtc agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct
      541 ctggctcgtt actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt
      601 gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa
      661 atcggtatcg aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct
      721 ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt ccgctgctaa cgacgaaaac
      781 tacgctctgg ttgcttaata actctgatag tgctagtgta gatctctact agagaaagag
      841 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      961 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1021 ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc
     1081 gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1321 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1441 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1561 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1681 ctcaccttcg ggtgggcctt tctgcgttta ta
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