LOCUS       BBa_K575032             1603 bp    DNA     linear       01-Jun-2024
DEFINITION  RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive 
            Promoter + RBS (B0030) + RhlR
ACCESSION   BBa_K575032
VERSION     BBa_K575032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     misc_feature    1..802
                     /label=BBa_K575012
     promoter        811..845
                     /label=BBa_J23119
     RBS             854..868
                     /label=RBS-1\Strong
     RBS             861..865
                     /label=RBS
     misc_feature    854..868
                     /label=BBa_B0030
     RBS             854..868
                     /label=BBa_B0030
     CDS             875..1603
                     /label=BBa_C0171
     misc_feature    811..1603
                     /label=BBa_K575006
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ttgacagcta gctcagtcct aggtataatg
      841 ctagctacta gagattaaag aggagaaata ctagatgagg aatgacggag gctttttgct
      901 gtggtgggac ggtttgcgta gcgagatgca gccgatccac gacagccagg gcgtgttcgc
      961 cgtcctggaa aaggaagtgc ggcgcctggg cttcgattac tacgcctatg gcgtgcgcca
     1021 cacgattccc ttcacccggc cgaagaccga ggtccatggc acctatccca aggcctggct
     1081 ggagcgatac cagatgcaga actacggggc cgtggatccg gcgatcctca acggcctgcg
     1141 ctcctcggaa atggtggtct ggagcgacag cctgttcgac cagagccgga tgctctggaa
     1201 cgaggctcgc gattggggcc tctgtgtcgg cgcgaccttg ccgatccgcg cgccgaacaa
     1261 tttgctcagc gtgctttccg tggcgcgcga ccagcagaac atctccagct tcgagcgcga
     1321 ggaaatccgc ctgcggctgc gttgcatgat cgagttgctg acccagaagc tgaccgacct
     1381 ggagcatccg atgctgatgt ccaacccggt ctgcctgagc catcgcgaac gcgagatcct
     1441 gcaatggacc gccgacggca agagttccgg ggaaatcgcc atcatcctga gcatctccga
     1501 gagcacggtg aacttccacc acaagaacat ccagaagaag ttcgacgcgc cgaacaagac
     1561 gctggctgcc gcctacgccg cggcgctggg tctcatctaa taa
//