LOCUS       BBa_K575033             1600 bp    DNA     linear       01-Jun-2024
DEFINITION  RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive 
            Promoter + RBS (B0034) + RhlR
ACCESSION   BBa_K575033
VERSION     BBa_K575033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     misc_feature    1..802
                     /label=BBa_K575012
     promoter        811..845
                     /label=BBa_J23119
     misc_feature    858..861
                     /label=conserved
     RBS             854..865
                     /label=BBa_B0034
     CDS             872..1600
                     /label=BBa_C0171
     misc_feature    811..1600
                     /label=BBa_K575007
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ttgacagcta gctcagtcct aggtataatg
      841 ctagctacta gagaaagagg agaaatacta gatgaggaat gacggaggct ttttgctgtg
      901 gtgggacggt ttgcgtagcg agatgcagcc gatccacgac agccagggcg tgttcgccgt
      961 cctggaaaag gaagtgcggc gcctgggctt cgattactac gcctatggcg tgcgccacac
     1021 gattcccttc acccggccga agaccgaggt ccatggcacc tatcccaagg cctggctgga
     1081 gcgataccag atgcagaact acggggccgt ggatccggcg atcctcaacg gcctgcgctc
     1141 ctcggaaatg gtggtctgga gcgacagcct gttcgaccag agccggatgc tctggaacga
     1201 ggctcgcgat tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt
     1261 gctcagcgtg ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg agcgcgagga
     1321 aatccgcctg cggctgcgtt gcatgatcga gttgctgacc cagaagctga ccgacctgga
     1381 gcatccgatg ctgatgtcca acccggtctg cctgagccat cgcgaacgcg agatcctgca
     1441 atggaccgcc gacggcaaga gttccgggga aatcgccatc atcctgagca tctccgagag
     1501 cacggtgaac ttccaccaca agaacatcca gaagaagttc gacgcgccga acaagacgct
     1561 ggctgccgcc tacgccgcgg cgctgggtct catctaataa
//