BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K575023 1 BBa_K575023 Genomic RhlR/PAI2 Inducible Promoter (short) 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z It is the upstream regulatory regions of (gene). This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-2/RhlR complex, part of the Pseudomonas quorum sensing hierarchy. false false _746_ 0 8746 9 It's complicated false This part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the regulatory region is. false Michael Vincent Sherer BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K575043 1 BBa_K575043 Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z from genome and registry Genomic Promoter with GFP reporter false false _746_ 0 8735 9 It's complicated false no false Kristin Palarz component2138515 1 BBa_K575023 component2138517 1 BBa_B0030 component2138520 1 BBa_E0040 annotation2138515 1 BBa_K575023 range2138515 1 1 521 annotation2138517 1 BBa_B0030 range2138517 1 530 544 annotation2138520 1 BBa_E0040 range2138520 1 551 1270 BBa_K575023_sequence 1 aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctc BBa_B0030_sequence 1 attaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K575043_sequence 1 aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z