LOCUS       BBa_K576012              994 bp    DNA     linear       10-May-2024
DEFINITION  J23101-RBS-GFP1-Lox-GFP2-Transcription Terminator
ACCESSION   BBa_K576012
VERSION     BBa_K576012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..535
                     /label=GFP1
     misc_feature    536..545
                     /label=MetaSuffix
     CDS             62..545
                     /label=BBa_K576001
     misc_feature    1..545
                     /label=BBa_K576005
     misc_feature    554..587
                     /label=BBa_J61046
     misc_feature    596..605
                     /label=MetaPrefix
     CDS             606..857
                     /label=GFP2
     CDS             596..857
                     /label=BBa_K576002
     stem_loop       877..920
                     /label=stem_loop
     misc_feature    866..945
                     /label=BBa_B0010
     terminator      866..945
                     /label=BBa_B0010
     stem_loop       961..980
                     /label=T7 TE
     misc_feature    987..987
                     /label=stop
     polyA_site      981..994
                     /label=polya
     misc_feature    954..994
                     /label=BBa_B0012
     terminator      954..994
                     /label=BBa_B0012
     terminator      866..994
                     /label=BBa_B0015
     misc_feature    596..994
                     /label=BBa_K576006
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaggttgaag
      541 agatctacta gagataactt cgtataatgt atgctatacg aagttattac tagaggagct
      601 cttcaatgaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      661 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      781 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      841 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      901 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      961 ggctcacctt cgggtgggcc tttctgcgtt tata
//