| Types | DnaRegion
|
| Roles | engineered_region
Measurement
|
| Sequences | BBa_K592028_sequence (Version 1)
|
Description
The promoter T5-lac (
BBa_K592008) tested for promoter strength coupled with YFP (
BBa_E0034). This construct should be cloned into pSB3K3 plasmid to measure the YFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.
Notes
B0032 was picked as per standard. The part was assembled into pSB3K3 for testing and cloned into pSB1C3 for DNA submission.
Source
T5-lac promoter is a fusion protein with parts from the T5 phage promoter.
