LOCUS       BBa_K598000             1007 bp    DNA     linear       11-Jun-2024
DEFINITION  TPP Down-regulated Hammerhead Ribozyme 2.5 with Native 
            RBS+E0040+B0015
ACCESSION   BBa_K598000
VERSION     BBa_K598000.1  
FEATURES             Location/Qualifiers
     RBS             138..145
                     /label=native RBS
     CDS             151..870
                     /label=eGFP
     misc_feature    879..958
                     /label=BBa_B0010
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    967..1007
                     /label=BBa_B0012
     stem_loop       974..993
                     /label=T7 TE
     polyA_site      994..1007
                     /label=polya
     misc_feature    1000..1000
                     /label=stop
     misc_feature    1..150
                     /label=TPP Ribozyme 2.5
     misc_feature    151..870
                     /label=BBa_E0040
     misc_feature    879..1007
                     /label=BBa_B0015
ORIGIN
        1 ttctccttcg gtacatccag ctgatgagtc ccaaatagga cgaaatcctc ggggtgccct
       61 tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg gataatgcca gcgtagggat
      121 ggatcctgga ttccacgaag gagatatacc atgcgtaaag gagaagaact tttcactgga
      181 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      241 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      301 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      361 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      421 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      481 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      541 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      601 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      661 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      721 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      781 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      841 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//