LOCUS       BBa_K598001              943 bp    DNA     linear       28-May-2024
DEFINITION  Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP 
            generator
ACCESSION   BBa_K598001
VERSION     BBa_K598001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..86
                     /label=Theophylline responsive riboswitch Theo 1G1
     RBS             75..81
                     /label=Engineered RBS
     misc_feature    87..806
                     /label=BBa_E0040
     CDS             87..806
                     /label=eGFP
     misc_feature    903..943
                     /label=BBa_B0012
     misc_feature    815..894
                     /label=BBa_B0010
     polyA_site      930..943
                     /label=polya
     misc_feature    936..936
                     /label=stop
     stem_loop       826..869
                     /label=stem_loop
     stem_loop       910..929
                     /label=T7 TE
ORIGIN
        1 tccatgtatg ttgatactta atttaaagat taaacaaaag atgataccag ccgaaaggcc
       61 cttggcagct ctcgaggagg tactagatgc gtaaaggaga agaacttttc actggagttg
      121 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      181 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      241 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      301 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      361 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      421 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      481 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      541 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
      601 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      661 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
      721 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      781 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
      841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      901 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//