LOCUS BBa_K598020 7064 bp DNA linear 11-May-2024 DEFINITION B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE ACCESSION BBa_K598020 VERSION BBa_K598020.1 FEATURES Location/Qualifiers stem_loop 1..129 /label=B0015 promoter 138..267 /label=pBAD RBS 273..279 /label=rbs CDS 285..1542 /label=vioA RBS 1545..1551 /label=rbs CDS 1559..4556 /label=vioB stem_loop 4565..4693 /label=B0015 promoter 4702..4831 /label=pBAD misc_feature 4838..4987 /label=TPP ribozyme 1.20 RBS 4976..4982 /label=rbs CDS 4988..6112 /label=vioD promoter 6342..6471 /label=pBAD stem_loop 6205..6333 /label=B0015 CDS 6489..7064 /label=vioE RBS 6477..6483 /label=rbs ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagaca ttgattattt gcacggcgtc acactttgct atgccatagc 181 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt 241 ttctccatac cgtttttttg ggctagcact agaggaggta ctagatgaaa cattcttccg 301 atatctgcat tgttggtgct ggtatttctg gtttgacgtg cgcaagccat ctgctggaca 361 gcccggcatg ccgtggtctg agcctgcgta tctttgacat gcagcaagaa gccggtggcc 421 gtatccgcag caaaatgctg gatggtaagg caagcattga actgggcgca ggtcgctact 481 cccctcagtt gcacccgcat ttccaaagcg caatgcagca ctatagccaa aagagcgaag 541 tctatccgtt cacccagttg aagttcaaat ctcacgtgca gcaaaagctg aagcgcgcca 601 tgaatgaact gtccccgcgt ctgaaagagc atggtaaaga gagctttttg cagtttgtca 661 gccgttatca aggtcacgat agcgcggttg gtatgatccg ctctatgggt tacgacgcac 721 tgttcctgcc ggatatcagc gcagaaatgg cctacgacat tgtgggtaag cacccggaga 781 tccagagcgt gacggacaac gacgcgaacc aatggtttgc agcggaaacg ggctttgctg 841 gtctgattca gggcatcaag gctaaggtta aggcggcagg tgcgcgtttt agcctgggtt 901 atcgtctgct gagcgtccgt accgacggtg acggctacct gctgcaactg gcaggtgacg 961 acggctggaa actggagcac cgtacccgcc atctgattct ggcgattccg ccgagcgcga 1021 tggcgggttt gaatgttgat tttccagaag cctggtccgg tgcgcgctat ggcagcctgc 1081 cgctgtttaa gggctttctg acgtacggtg agccgtggtg gttggactac aaactggacg 1141 atcaggtgct gattgttgac aacccgctgc gcaaaatcta tttcaaaggc gataagtacc 1201 tgttcttcta taccgatagc gagatggcga attactggcg cggttgtgtc gcggagggcg 1261 aggacggtta cctggagcaa attcgcaccc atttggctag cgcactgggt atcgtccgtg 1321 aacgtatccc gcaaccgctg gcacacgttc acaagtattg ggcgcacggc gttgagtttt 1381 gccgtgattc tgatattgac cacccgagcg cactgtctca tcgcgacagc ggtatcatcg 1441 cgtgctccga tgcgtacacg gagcattgtg gttggatgga gggcggtctg ctgagcgccc 1501 gtgaggcaag ccgtctgctg ttgcagcgta tcgccgcgtg attaaggagg taaaaaaaat 1561 gagcattctg gatttcccgc gtatccactt ccgtggctgg gcccgtgtca atgcgccgac 1621 cgcgaaccgc gatccgcacg gccacatcga tatggccagc aataccgtgg cgatggcggg 1681 tgagccgttc gacctggcac gccatcctac ggagttccac cgtcacctgc gctccctggg 1741 tccgcgcttc ggcttggatg gtcgtgctga cccggaaggc ccgttcagcc tggccgaggg 1801 ctacaacgct gccggtaaca accacttttc gtgggagagc gcaaccgtta gccacgtgca 1861 atgggatggc ggtgaggcgg atcgtggtga cggtctggtc ggtgctcgtt tggcactgtg 1921 gggtcactac aatgattatc tgcgtaccac cttcaatcgt gctcgttggg tcgacagcga 1981 cccgacgcgc cgtgacgctg cacaaatcta tgcgggccaa ttcaccatta gcccggctgg 2041 tgccggtccg ggtacgccgt ggctgtttac ggcagacatt gatgatagcc atggtgcacg 2101 ttggacgcgt ggcggccaca ttgcagagcg tggcggccac ttcttggatg aagagtttgg 2161 tctggcacgc ctgtttcagt tctctgtgcc gaaagatcac ccacattttc tgtttcaccc 2221 gggtccgttt gattccgagg cctggcgtcg tctgcaattg gctctggagg atgacgacgt 2281 tctgggtctg accgtgcaat atgcgttgtt caatatgagc accccgcctc agccgaacag 2341 cccggttttt cacgatatgg tcggtgttgt cggtctgtgg cgtcgtggtg aactggcgag 2401 ctacccggct ggtcgtctgc tgcgtccgcg tcaaccgggt ctgggtgacc tgaccctgcg 2461 cgtcaacggt ggtcgcgttg cgctgaattt ggcgtgtgcc attccgttca gcactcgtgc 2521 cgcgcagcca agcgcaccgg accgcctgac cccggacctg ggtgccaaac tgccgctggg 2581 cgatctgctg ctgcgtgatg aggacggcgc actgttggca cgtgtgccgc aggctctgta 2641 ccaagactat tggacgaatc acggtattgt ggacctgccg ctgctgcgcg aaccgcgtgg 2701 tagcttgacc ctgagcagcg aactggcgga gtggcgtgag caagactggg tcacccaaag 2761 cgacgcgtct aacctgtacc tggaggcacc ggatcgccgt cacggtcgct ttttccctga 2821 gagcatcgcg ctgcgcagct actttcgcgg tgaagcgcgt gcgcgtccgg atatcccgca 2881 tcgtatcgag ggcatgggcc tggtcggcgt cgaatctcgt caggatggcg acgctgcgga 2941 atggcgtctg acgggtctgc gtccgggtcc ggcacgcatt gttctggacg atggtgccga 3001 ggcgatccct ctgcgtgttc tgcctgacga ttgggcgctg gatgacgcga ccgtcgaaga 3061 agtggattac gcctttttgt accgccacgt tatggcgtat tacgagctgg tgtatccatt 3121 catgagcgac aaggtgtttt ccctggctga tcgttgcaaa tgtgaaacgt acgcacgtct 3181 gatgtggcag atgtgtgatc cgcagaaccg caacaagtcc tattacatgc cgagcacccg 3241 cgaactgtcg gcaccgaaag ctcgtttgtt cttgaagtat ctggcccacg tggaaggcca 3301 ggcacgcctg caagcacctc cgccagcggg tccggcacgc attgaatcta aagcccagtt 3361 ggcggcagag ctgcgtaaag ccgtcgacct ggagctgtct gtgatgctgc aatacctgta 3421 cgcggcgtat agcattccga actatgcaca gggccaacaa cgtgttcgtg acggtgcgtg 3481 gaccgccgag cagctgcaac tggcgtgcgg tagcggtgac cgtcgccgtg atggcggtat 3541 tcgtgcagca ctgctggaaa ttgctcatga agaaatgatt cattacctgg tcgttaacaa 3601 cctgctgatg gccctgggcg agccgttcta cgcgggtgtc ccgctgatgg gcgaagcggc 3661 acgtcaggcg tttggcctgg acaccgagtt cgctctggaa ccgtttagcg aaagcacgct 3721 ggcacgtttt gttcgtctgg aatggccgca ctttatccca gcaccgggca aatccatcgc 3781 ggactgctat gccgccattc gtcaggcgtt tttggatctg ccggacttgt ttggtggcga 3841 ggcaggtaag cgtggcggtg aacaccacct gttcctgaat gagctgacca accgtgcgca 3901 tccgggttat caactggaag ttttcgatcg cgactcggcg ctgtttggta ttgcatttgt 3961 gaccgatcag ggcgaaggtg gcgctctgga cagcccgcac tacgaacata gccattttca 4021 acgtctgcgt gaaatgagcg cgcgtatcat ggctcaaagc gcaccgttcg aaccggcgct 4081 gccggcgttg cgtaatccgg ttctggatga gagcccgggt tgccaacgtg tcgcagacgg 4141 tcgtgcgcgt gcgctgatgg cattgtacca aggcgtttat gagctgatgt ttgcgatgat 4201 ggcgcagcac ttcgccgtga aaccgctggg tagcttgcgt cgcagccgcc tgatgaacgc 4261 agcaatcgat ctgatgaccg gtctgttgcg tccgctgagc tgcgcgctga tgaacctgcc 4321 aagcggcatc gccggtcgca cggccggtcc gccgctgccg ggtccggttg acacccgtag 4381 ctatgacgac tacgcgctgg gctgtcgcat gctggcacgc cgttgcgagc gtctgctgga 4441 gcaggcgagc atgctggaac cgggttggct gccggatgcg cagatggagc tgctggattt 4501 ctatcgtcgc caaatgctgg acttggcgtg cggcaaactg agccgcgagg cctaagtact 4561 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4621 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4681 ttctgcgttt atatactaga gacattgatt atttgcacgg cgtcacactt tgctatgcca 4741 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta 4801 ctgtttctcc ataccgtttt tttgggctag cactagattc tccttcggta catccagctg 4861 atgagtccca aataggacga aaacatcggg gtgcccttct gcgtgaaggc tgagaaatac 4921 ccgtatcacc tgatctggat aatgccagcg tagggattat tcctggattc cacgaaggag 4981 atataccatg aagattctgg tcattggtgc tggtccagct ggtctggttt tcgcatccca 5041 actgaagcag gcacgccctt tgtgggccat tgacatcgtg gagaagaatg acgagcaaga 5101 agtgctgggc tggggtgtcg tgctgcctgg ccgtccgggt cagcacccgg cgaacccgct 5161 gtcctatctg gatgcaccgg agcgtctgaa tccgcaattt ctggaggact tcaaactggt 5221 gcatcataat gagccgtcct tgatgtccac gggcgttttg ttgtgcggcg tggagcgtcg 5281 cggtctggtt cacgcgctgc gcgataagtg ccgcagccaa ggcattgcta ttcgtttcga 5341 aagcccgttg ctggaacacg gtgagctgcc gctggcggac tatgatctgg tggtcctggc 5401 taatggtgtt aatcacaaaa ccgcgcattt caccgaggct ctggtcccgc aggtggacta 5461 cggccgcaat aagtacattt ggtatggcac tagccagctg ttcgatcaga tgaatctggt 5521 ttttcgtacc catggtaaag atatctttat cgcgcatgcc tataagtata gcgataccat 5581 gagcacgttc attgtcgaat gtagcgaaga gacttacgca cgcgcacgcc tgggcgaaat 5641 gtccgaagag gcgagcgcag aatacgttgc taaggtgttc caggccgagc tgggtggtca 5701 cggcctggtg agccagccgg gtctgggttg gcgtaacttc atgacgttgt ctcatgaccg 5761 ttgtcatgat ggtaagttgg ttctgctggg tgacgcgctg caaagcggtc actttagcat 5821 cggccacggc accacgatgg ccgtggtggt ggcgcagctg ctggttaaag cgctgtgtac 5881 cgaagatggt gtgcctgccg cgctgaaacg tttcgaagag cgtgccctgc cgctggtgca 5941 gttgttccgt ggccacgcag acaacagccg cgtttggttc gaaaccgtcg aagagcgcat 6001 gcacctgtcc tcggcggaat ttgtgcaaag cttcgacgca cgccgcaaaa gcctgccgcc 6061 gatgccggaa gcactggcgc agaatctgcg ttatgctttg cagcgctgat gattcattaa 6121 ggaggtaaaa aaaatggaga accgtgagcc accactgttg ccagcccgtt ggagcagcgc 6181 ctatgtctct tattggtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 6241 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 6301 gctcaccttc gggtgggcct ttctgcgttt atatactaga gacattgatt atttgcacgg 6361 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg 6421 ctttttatcg caactctcta ctgtttctcc ataccgtttt tttgggctag cactagagga 6481 ggtactagat ggagaaccgt gagccaccac tgttgccagc ccgttggagc agcgcctatg 6541 tctcttattg gagcccgatg ctgccggatg accagctgac cagcggctat tgctggttcg 6601 actatgaacg tgacatctgt cgtattgacg gcctgttcaa tccgtggagc gagcgtgata 6661 ctggttatcg cctgtggatg tcggaggttg gtaatgcggc cagcggccgt acctggaaac 6721 aaaaagtcgc ctatggtcgt gagcgtaccg ccctgggtga acagctgtgt gagcgtccgc 6781 tggatgatga gactggccct tttgccgaat tgttcctgcc acgcgatgtc ctgcgccgtc 6841 tgggtgcccg tcacattggc cgtcgcgtgg ttctgggtcg cgaagcggac ggttggcgtt 6901 accagcgccc aggtaaaggt ccgagcaccc tgtacctgga tgcggcgagc ggcactccac 6961 tgcgcatggt caccggcgat gaagcgtcgc gtgcaagcct gcgtgatttt ccgaatgtga 7021 gcgaggcgga gatcccggac gcggttttcg cggccaagcg ctaa //