Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan GONG, Shuobin CHEN, Yunpeng LIU
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:50
Bistable Switch Mutant 42
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K598022_sequence (Version 1) |
Description
The RBS of CI434 is CGAGACC in this bistble switch, which makes the RFP expresses three times as the GFP does in this bistable switch.Notes
The library is constructed by mutagenesis.Source
Engineered| Sequence Annotation | Location | Component / Role(s) |
| mRFP RBS lamda CI repressor RBS PRM (CI434 regulated promoter) PR (CI regulated promoter) RBS CI434 repressor RBS eGFP | 1,681 682,693 708,1421 1422,1433 1440,1491 1492,1540 1556,1567 1568,2203 2214,2225 2226,2945 | feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs promoter feature/promoter promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds feature/rbs ribosome_entry_site feature/cds CDS |