LOCUS BBa_K606003 5924 bp DNA linear 29-May-2024 DEFINITION Multi-host replicative plasmid, for B. Subtilis and E. Coli ACCESSION BBa_K606003 VERSION BBa_K606003.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick EX misc_feature 22..3072 /label=pBC16-1 plasmid (B.S. plasmid) CDS 80..1468 /label=Tetracycline resistance gene misc_feature 2120..2398 /label=DNA minor replication origin OriL misc_feature 2813..2838 /label=DNA: Major Replication origin OriU CDS 3076..3244 /label=LacZ alpha disabled by the cloning misc_feature 3078..5904 /label=SYNPTZ19R CDS 3835..4692 /label=Beta lactamase peptide (ampicilin) misc_feature 4867..5469 /label=Replication origin for E. Coli promoter 5871..5893 /label=pT7 promoter misc_feature 5905..5924 /label=Biobrick SP ORIGIN 1 ctctagaagc ggccgcgaat tcctgttata aaaaaaggat caattttgaa ctctctccca 61 aagttgatcc cttaacgatt tagaaatccc tttgagaatg tttatataca ttcaaggtaa 121 ccagccaact aatgacaatg attcctgaaa aaagtaataa caaattacta tacagataag 181 ttgactgatc aacttccata ggtaacaacc tttgatcaag taagggtatg gataataaac 241 cacctacaat tgcaatacct gttccctctg ataaaaagct ggtaaagtta agcaaactca 301 ttccagcacc agcttcctgc tgtttcaagc tacttgaaac aattgttgat ataactgttt 361 tggtgaacga aagcccacct aaaacaaata cgattataat tgtcatgaac catgatgttg 421 tttctaaaag aaaggaagca gttaaaaagc taacagaaag aaatgtaact ccgatgttta 481 acacgtataa aggacctctt ctatcaacaa gtatcccacc aatgtagccg aaaataatga 541 cactcattgt tccagggaaa ataattacac ttccgatttc ggcagtactt agctggtgaa 601 catctttcat catataagga accatagaga caaaccctgc tactgttcca aatataattc 661 ccccacaaag aactccaatc ataaaaggta tatttttccc taatccggga tcaacaaaag 721 gatctgttac tttcctgata tgttttacaa atatcaggaa tgacagcacg ctaacgataa 781 gaaaagaaat gctatatgat gttgtaaaca acataaaaaa tacaatgcct acagacatta 841 gtataattcc tttgatatca aaatgacctt ttatccttac ttctttcttt aataatttca 901 taagaaacgg aacagtgata attgttatca taggaatgag tagaagatag gaccaatgaa 961 tataatgggc tatcattcca ccaatcgctg gaccgactcc ttctcccatg gctactatcg 1021 atccaataag accaaatgct ttacccctat tttcctttgg aatatagcgc gcaactacaa 1081 ccattacgag tgctggaaat gcagctgcac cagccccttg aataaaacga gccataataa 1141 gtaaggaaaa gaaagaatgg ccaacaaacc caattaccga cccgaaacaa tttattataa 1201 ttccaaatag gagtaacctt ttgatgccta attgatcaga tagctttcca tatacagctg 1261 ttccaatgga aaagcttaac ataaaggctg tgttcaccca gtttgtactc gcagctggtt 1321 tattaaaatc atttgcaata tcaggtaatg agacgttcaa aaccatttca tttaatacgc 1381 taaaaaaaga taaaatgcaa agccaaatta aaatttggtt gtgtcgtaaa ttcgattgtg 1441 aataggatgt attcacattt caccctccaa taatgagggc agacgtagtt tatacggtta 1501 atgatacgct tccctctttt aattgaaccc tgttacattc attacacttc ataattaatt 1561 cctcctaaac ttgattaaaa cattttacca catataaact aagttttaaa ttcagtattt 1621 catcacttat acaacaatat ggcccgtttg ttgaactact ctttaataaa ataatttttc 1681 cgttcccaat tccacattgc aataatagaa aatccatctt catccgcttt ttcgtcatca 1741 tctgtatgaa tcaaatcgcc ttcttctgtg tcatcaaggt ttaatttttt atgtatttct 1801 tttaacaaac caccatagga gattaacctt ttacggtgta aaccttcctc caaatcagac 1861 aaacgtttca aattcttttc ttcatcatcg gtcataaaat ccgtatcctt tacagcatat 1921 tttgcagttt cgtcaattcc cgattgtata tccgatttat atttattttt cggtcgaatc 1981 atttgaactt ttacatttgg atcatagtct aatttcattg cctttttcca aaattgaatc 2041 cattgttttt gattcacgta gttttctcta ttcttaaaat aagttggttc cacacatacc 2101 aatacatcca tgtgctcatt ataagaatta tctttattat ttattgtcac ttccgttcca 2161 cgcataaaac caacaagatt tttattaatt tttttatatt gcatcattcg gcgaaatcct 2221 tgagccatat ctgacaaact cttatttaat tcttcgccat cataaacatt tttaactgtt 2281 aatgtgagaa acaaccaacg aactgttgcc ttttgtttaa taacttcagc aacaaccttt 2341 tgtgactgaa tgccatgttt cattgctctc ctccagttgc acattggaca aagcctggat 2401 ttacaaaacc acactcgata caactttctt tcgcctgttt cacgattttg tttatactct 2461 aatatttcag cacaatcttt tactctttca gcctttttaa attcaacaat atgcagaact 2521 tcaaagtaat caacattagc gattttcttt tctctccatg gtctcacttt tccacttttt 2581 gtcttgtcca ctaaaaccct tgatttttca tctgaataaa tgctactatt aggacacata 2641 atattaaaag aaacccccat ctatttagtt atttgtttgg tcacttataa ctttaacaga 2701 tggggttttt ctgtgcaacc aattttaagc gttttcaata ctttaaaaca catacatacc 2761 aacacttcaa cgcacctttc agcaactaaa ataaaaatga cgttatttct atatgtatca 2821 agataagaaa gaacaagttc aaaaccatca aaaaaagaca ccttttcagc tgcttttttt 2881 attttataaa ctcattccct gatctccact tcgttctttt tttacctctc ggttatgagt 2941 tagttcaaat tcgttctttt taggttctaa atcgtgtttt tcttggaatt gtgctgtttt 3001 atcctttacc ttgtctacaa accccttaaa aacgttttta aaggctttta agccgtctgt 3061 acgttcctta agggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 3121 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 3181 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat 3241 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 3301 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 3361 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 3421 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 3481 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 3541 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 3601 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 3661 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg 3721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 3781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 3841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 4021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 4081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 4141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 4201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 4261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 4321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 4381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 4441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 4501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 4561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 4621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 4741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4981 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 5041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 5521 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 5581 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 5641 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 5701 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 5761 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 5821 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt taatacgact 5881 cactataggg aaagcttgca tgcctgcagc ggccgctact agta //