LOCUS       BBa_K606014              875 bp    DNA     linear       05-May-2024
DEFINITION  RBS SpoVG GFPmut3b (reporter system for B. Subtilis)
ACCESSION   BBa_K606014
VERSION     BBa_K606014.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=rbs
     RBS             1..12
                     /label=BBa_K143021
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
ORIGIN
        1 aaaggtggtg aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttata
//