GFP:LacI

BBa_K606020 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K606020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: K??vin Yauy

Date created: 2011-07-26 11:00:00

Date modified: 2015-05-08 01:12:51

GFP:LacI protein fusion

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K606020_sequence (Version 1)

Description

GFP:LacI protein fusion.
Use to focalise fluorescence in few spots.
It binds on LacO array (BBa_K606021) (containing 10kb of LacO).

We use it to prove the existence of nanotube between B.subtilis as a proof of concept.

Notes

2 Quick Change Mutagenesis on the basic sequence has been done

Source

Andrew Wright, Dave Lane, pSG20 plasmid.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,1788	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K606020/1

Member of these Collections

• iGEM Parts Registry