LOCUS       BBa_K606059              980 bp    DNA     linear       01-Jun-2024
DEFINITION  pVeg SpoVG GFPmut3b tyrosine amber double terminator
ACCESSION   BBa_K606059
VERSION     BBa_K606059.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     misc_feature    321..321
                     /label=amber mutation
     CDS             124..840
                     /label=GFP amber
     misc_feature    841..843
                     /label=stop
     CDS             124..843
                     /label=BBa_K606043
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
     terminator      852..980
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      181 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      241 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      301 cttgtcacta ctttcggtta gggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      361 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      421 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      481 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      541 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      601 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      661 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      721 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      781 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata
//