LOCUS       BBa_K607012             1269 bp    DNA     linear       12-May-2024
DEFINITION  Prm_cI-LVA_antiPlasB with 0.6RBS
ACCESSION   BBa_K607012
VERSION     BBa_K607012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     RBS             228..242
                     /label=RBS-1\Strong
     RBS             235..239
                     /label=RBS
     misc_feature    228..242
                     /label=BBa_B0030
     RBS             228..242
                     /label=BBa_B0030
     CDS             252..959
                     /label=cI lambda
     misc_feature    960..992
                     /label=LVA
     misc_feature    999..1023
                     /label=Help:Barcodes
     CDS             249..1023
                     /label=BBa_C0051
     misc_feature    1032..1132
                     /label=misc
     promoter        1032..1132
                     /label=BBa_K607008
     stem_loop       1152..1195
                     /label=stem_loop
     misc_feature    1141..1220
                     /label=BBa_B0010
     terminator      1141..1220
                     /label=BBa_B0010
     stem_loop       1236..1255
                     /label=T7 TE
     misc_feature    1262..1262
                     /label=stop
     polyA_site      1256..1269
                     /label=polya
     misc_feature    1229..1269
                     /label=BBa_B0012
     terminator      1229..1269
                     /label=BBa_B0012
     terminator      1141..1269
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagatt aaagaggaga
      241 aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc
      301 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg
      361 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat
      421 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta
      481 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac
      541 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg
      601 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca
      661 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca
      721 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag
      781 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg
      841 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg
      901 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg
      961 ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat
     1021 cgctactaga ggctttcgtg taccaaaaag aacccgcggc caaacctgcc agaactggca
     1081 ggtagccttg atttcgccgg aaatctgtat gttttcgctg gaatagctag catactagag
     1141 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1201 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1261 gcgtttata
//