LOCUS       BBa_K607016             1244 bp    DNA     linear       09-May-2024
DEFINITION  Prm_cI-DAS_antiPlasB with 0.3RBS
ACCESSION   BBa_K607016
VERSION     BBa_K607016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     RBS             228..240
                     /label=RBS-3\Weak
     RBS             234..237
                     /label=RBS
     misc_feature    228..240
                     /label=BBa_B0032
     RBS             228..240
                     /label=BBa_B0032
     misc_feature    247..957
                     /label=BBa_K607015
     misc_feature    966..998
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    966..998
                     /label=BBa_M0052
     misc_feature    1007..1107
                     /label=misc
     promoter        1007..1107
                     /label=BBa_K607008
     stem_loop       1127..1170
                     /label=stem_loop
     misc_feature    1116..1195
                     /label=BBa_B0010
     terminator      1116..1195
                     /label=BBa_B0010
     stem_loop       1211..1230
                     /label=T7 TE
     misc_feature    1237..1237
                     /label=stop
     polyA_site      1231..1244
                     /label=polya
     misc_feature    1204..1244
                     /label=BBa_B0012
     terminator      1204..1244
                     /label=BBa_B0012
     terminator      1116..1244
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagtca cacaggaaag
      241 tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc
      301 cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca
      361 gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta
      421 aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc
      481 ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt
      541 agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag
      601 cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt
      661 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag
      721 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt
      781 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat
      841 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag
      901 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggctac
      961 tagaggctgc taacgacgaa aactacgctg acgcttctta ctagaggctt tcgtgtacca
     1021 aaaagaaccc gcggccaaac ctgccagaac tggcaggtag ccttgatttc gccggaaatc
     1081 tgtatgtttt cgctggaata gctagcatac tagagccagg catcaaataa aacgaaaggc
     1141 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1201 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//