LOCUS       BBa_K607017             1246 bp    DNA     linear       13-May-2024
DEFINITION  Prm_cI-DAS_antiPlasB with 0.6RBS
ACCESSION   BBa_K607017
VERSION     BBa_K607017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     RBS             228..242
                     /label=RBS-1\Strong
     RBS             235..239
                     /label=RBS
     misc_feature    228..242
                     /label=BBa_B0030
     RBS             228..242
                     /label=BBa_B0030
     misc_feature    249..959
                     /label=BBa_K607015
     misc_feature    968..1000
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    968..1000
                     /label=BBa_M0052
     misc_feature    1009..1109
                     /label=misc
     promoter        1009..1109
                     /label=BBa_K607008
     stem_loop       1129..1172
                     /label=stem_loop
     misc_feature    1118..1197
                     /label=BBa_B0010
     terminator      1118..1197
                     /label=BBa_B0010
     stem_loop       1213..1232
                     /label=T7 TE
     misc_feature    1239..1239
                     /label=stop
     polyA_site      1233..1246
                     /label=polya
     misc_feature    1206..1246
                     /label=BBa_B0012
     terminator      1206..1246
                     /label=BBa_B0012
     terminator      1118..1246
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagatt aaagaggaga
      241 aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc
      301 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg
      361 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat
      421 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta
      481 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac
      541 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg
      601 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca
      661 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca
      721 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag
      781 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg
      841 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg
      901 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggct
      961 actagaggct gctaacgacg aaaactacgc tgacgcttct tactagaggc tttcgtgtac
     1021 caaaaagaac ccgcggccaa acctgccaga actggcaggt agccttgatt tcgccggaaa
     1081 tctgtatgtt ttcgctggaa tagctagcat actagagcca ggcatcaaat aaaacgaaag
     1141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1201 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//