LOCUS       BBa_K607018             1243 bp    DNA     linear       13-May-2024
DEFINITION  Prm_cI-DAS_antiPlasB with 1.0RBS
ACCESSION   BBa_K607018
VERSION     BBa_K607018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     misc_feature    232..235
                     /label=conserved
     RBS             228..239
                     /label=BBa_B0034
     misc_feature    246..956
                     /label=BBa_K607015
     misc_feature    965..997
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    965..997
                     /label=BBa_M0052
     misc_feature    1006..1106
                     /label=misc
     promoter        1006..1106
                     /label=BBa_K607008
     stem_loop       1126..1169
                     /label=stem_loop
     misc_feature    1115..1194
                     /label=BBa_B0010
     terminator      1115..1194
                     /label=BBa_B0010
     stem_loop       1210..1229
                     /label=T7 TE
     misc_feature    1236..1236
                     /label=stop
     polyA_site      1230..1243
                     /label=polya
     misc_feature    1203..1243
                     /label=BBa_B0012
     terminator      1203..1243
                     /label=BBa_B0012
     terminator      1115..1243
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagaaa gaggagaaat
      241 actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
      301 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
      361 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
      421 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
      481 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
      541 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
      601 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
      661 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
      721 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg
      781 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata
      841 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga
      901 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctact
      961 agaggctgct aacgacgaaa actacgctga cgcttcttac tagaggcttt cgtgtaccaa
     1021 aaagaacccg cggccaaacc tgccagaact ggcaggtagc cttgatttcg ccggaaatct
     1081 gtatgttttc gctggaatag ctagcatact agagccaggc atcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1201 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//