LOCUS       BBa_K607027             2441 bp    DNA     linear       11-May-2024
DEFINITION  PlasB_LasR-DAS_antipBad/araC with 0.07RBS
ACCESSION   BBa_K607027
VERSION     BBa_K607027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     misc_feature    309..312
                     /label=conserved
     RBS             303..316
                     /label=BBa_B0031
     CDS             323..1045
                     /label=BBa_C0179
     misc_feature    1054..1086
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    1054..1086
                     /label=BBa_M0052
     promoter        1095..2304
                     /label=BBa_K607040
     stem_loop       2324..2367
                     /label=stem_loop
     misc_feature    2313..2392
                     /label=BBa_B0010
     terminator      2313..2392
                     /label=BBa_B0010
     stem_loop       2408..2427
                     /label=T7 TE
     misc_feature    2434..2434
                     /label=stop
     polyA_site      2428..2441
                     /label=polya
     misc_feature    2401..2441
                     /label=BBa_B0012
     terminator      2401..2441
                     /label=BBa_B0012
     terminator      2313..2441
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agtcacacag gaaacctact agatggcctt ggttgacggt tttcttgagc tggaacgctc
      361 aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc
      421 gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt
      481 cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga
      541 cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta
      601 ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg
      661 gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga
      721 agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat
      781 gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa
      841 accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac
      901 cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg
      961 aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa
     1021 tttgggtctt attactctct aataatacta gaggctgcta acgacgaaaa ctacgctgac
     1081 gcttcttact agaggctagc ccaaaaaaac gggtatggag aaacagtaga gagttgcgat
     1141 aaaaagcgtc aggtaggatc cgctaatctt atggataaaa atgctatggc atagcaaagt
     1201 gtgacgccgt gcaaataatc aatgtggact tttctgccgt gattatagac acttttgtta
     1261 cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa tgcttttaat aagcggggtt
     1321 accggtttgg ttagcgagaa gagccagtaa aagacgcagt gacggcaatg tctgatgcaa
     1381 tatggacaat tggtttcttc tctgaatggc gggagtatga aaagtatggc tgaagcgcaa
     1441 aatgatcccc tgctgccggg atactcgttt aatgcccatc tggtggcggg tttaacgccg
     1501 attgaggcca acggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat
     1561 attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtt
     1621 tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat
     1681 ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat
     1741 gaatggctta actggccgtc aatatttgcc aatacggggt tctttcgccc ggatgaagcg
     1801 caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg
     1861 cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa
     1921 gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac
     1981 atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc
     2041 ttgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc
     2101 tggcgcgagg accaacgtat cagccaggcg aagctgcttt tgagcaccac ccggatgcct
     2161 atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgggtattt
     2221 aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat
     2281 gatgtagccg tcaagttgtc ataatactag agccaggcat caaataaaac gaaaggctca
     2341 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2401 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//