LOCUS       BBa_K607030             2439 bp    DNA     linear       10-May-2024
DEFINITION  PlasB_LasR-DAS_antipBad/araC with 1.0RBS
ACCESSION   BBa_K607030
VERSION     BBa_K607030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     misc_feature    307..310
                     /label=conserved
     RBS             303..314
                     /label=BBa_B0034
     CDS             321..1043
                     /label=BBa_C0179
     misc_feature    1052..1084
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    1052..1084
                     /label=BBa_M0052
     promoter        1093..2302
                     /label=BBa_K607040
     stem_loop       2322..2365
                     /label=stem_loop
     misc_feature    2311..2390
                     /label=BBa_B0010
     terminator      2311..2390
                     /label=BBa_B0010
     stem_loop       2406..2425
                     /label=T7 TE
     misc_feature    2432..2432
                     /label=stop
     polyA_site      2426..2439
                     /label=polya
     misc_feature    2399..2439
                     /label=BBa_B0012
     terminator      2399..2439
                     /label=BBa_B0012
     terminator      2311..2439
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      361 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      421 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      481 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      541 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      601 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      661 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      721 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      781 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      841 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      901 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      961 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
     1021 tgggtcttat tactctctaa taatactaga ggctgctaac gacgaaaact acgctgacgc
     1081 ttcttactag aggctagccc aaaaaaacgg gtatggagaa acagtagaga gttgcgataa
     1141 aaagcgtcag gtaggatccg ctaatcttat ggataaaaat gctatggcat agcaaagtgt
     1201 gacgccgtgc aaataatcaa tgtggacttt tctgccgtga ttatagacac ttttgttacg
     1261 cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa gcggggttac
     1321 cggtttggtt agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata
     1381 tggacaattg gtttcttctc tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa
     1441 tgatcccctg ctgccgggat actcgtttaa tgcccatctg gtggcgggtt taacgccgat
     1501 tgaggccaac ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat
     1561 tctcaatctc accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtttg
     1621 ccgaccgggt gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc
     1681 ggaggctcgc gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga
     1741 atggcttaac tggccgtcaa tatttgccaa tacggggttc tttcgcccgg atgaagcgca
     1801 ccagccgcat ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg
     1861 ctattcggag ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc
     1921 gattaacgag tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat
     1981 cagcgatcac ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgctt
     2041 gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg
     2101 gcgcgaggac caacgtatca gccaggcgaa gctgcttttg agcaccaccc ggatgcctat
     2161 cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gggtatttaa
     2221 aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga
     2281 tgtagccgtc aagttgtcat aatactagag ccaggcatca aataaaacga aaggctcagt
     2341 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2401 acactggctc accttcgggt gggcctttct gcgtttata
//