LOCUS       BBa_K607032             2399 bp    DNA     linear       10-May-2024
DEFINITION  PlasB_LasR_antipBad/araC with 0.3RBS
ACCESSION   BBa_K607032
VERSION     BBa_K607032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     RBS             303..315
                     /label=RBS-3\Weak
     RBS             309..312
                     /label=RBS
     misc_feature    303..315
                     /label=BBa_B0032
     RBS             303..315
                     /label=BBa_B0032
     CDS             322..1044
                     /label=BBa_C0179
     promoter        1053..2262
                     /label=BBa_K607040
     stem_loop       2282..2325
                     /label=stem_loop
     misc_feature    2271..2350
                     /label=BBa_B0010
     terminator      2271..2350
                     /label=BBa_B0010
     stem_loop       2366..2385
                     /label=T7 TE
     misc_feature    2392..2392
                     /label=stop
     polyA_site      2386..2399
                     /label=polya
     misc_feature    2359..2399
                     /label=BBa_B0012
     terminator      2359..2399
                     /label=BBa_B0012
     terminator      2271..2399
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agtcacacag gaaagtacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      361 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      421 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      481 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      541 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      601 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      661 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      721 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      781 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      841 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      901 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      961 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
     1021 ttgggtctta ttactctcta ataatactag aggctagccc aaaaaaacgg gtatggagaa
     1081 acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat
     1141 gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga
     1201 ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg
     1261 cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga
     1321 cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa
     1381 agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg
     1441 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg
     1501 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat
     1561 cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc aggagagatt
     1621 catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt
     1681 ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa tacggggttc
     1741 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac
     1801 gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg
     1861 ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta
     1921 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc
     1981 gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta
     2041 gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa gctgcttttg
     2101 agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc
     2161 tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt
     2221 tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aatactagag ccaggcatca
     2281 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2341 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//