LOCUS       BBa_K611073             2998 bp    DNA     linear       01-Jun-2024
DEFINITION  LVA 5' -22bp Truncation (BBa_K611096) test construct
ACCESSION   BBa_K611073
VERSION     BBa_K611073.1  
FEATURES             Location/Qualifiers
     promoter        2728..2756
                     /label=beta-lactamase (ampR, bla) promoter
     CDS             27..707
                     /label=mRFP
     misc_feature    741..869
                     /label=BBa_B0015
     misc_feature    1049..1731
                     /label=ColE1 origin
     CDS             1826..2686
                     /label=beta-lactamase ampicillin resistance marker
     stem_loop       752..795
                     /label=rrnB terminator
     misc_feature    1052..1671
                     /label=pBR322 origin
     misc_feature    2927..2948
                     /label=BioBrick prefix
     misc_feature    2..7
                     /label=SpeI
     misc_feature    885..890
                     /label=PstI
     misc_feature    878..885
                     /label=NotI
     RBS             9..20
                     /label=RBS
     misc_feature    9..20
                     /label=BBa_B0034 RBS
     primer_bind     948..967
                     /label=Verification reverse (VR) primer binding site
     primer_bind     2809..2828
                     /label=Verification forward (VF2) primer binding site
     misc_feature    2941..2941
                     /label=Ambiguity; should be a T
     misc_feature    1..2948
                     /label=BBa_J61002
     misc_feature    2957..2967
                     /label=LVA 5' -22bp
     misc_feature    2974..2998
                     /label=Barcode
     misc_feature    2957..2998
                     /label=BBa_K611096
ORIGIN
        1 tactagtgaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca
       61 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      121 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      181 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      241 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      301 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
      361 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      421 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      481 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      541 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      601 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
      661 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct
      721 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      841 accttcgggt gggcctttct gcgtttatat actagaagcg gccgctgcag gcttcctcgc
      901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2821 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     2881 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     2941 atctagagta ctagagcttt agtagcttaa taacgctgat agtgctagtg tagatcgc
//